Sarkar D P, Blumenthal R
Section on Membrane Structure and Function, National Cancer Institute, Bethesda, MD 20892.
Membr Biochem. 1987;7(4):231-47. doi: 10.3109/09687688709029434.
Fusion between membranes of Sendai virus and liposomes or human erythrocytes ghosts was studied using an assay for lipid mixing based on the relief of self-quenching of octadecylrhodamine (R18) fluorescence. We considered only viral fusion that reflects the biological activity of the viral spike glycoproteins. The liposomes were made of phosphatidylcholine, and the effects of including cholesterol, the sialoglycolipid GD1a, and/or the sialoglycoprotein glycophorin as receptors were tested. Binding of Sendai virus to those liposomes at 37 degrees C was very weak. Fusion with the erythrocyte membranes occurred at a 30-fold faster rate than with the liposomes. Experiments with biological and liposomal targets of different size indicated that size did not account for differences in fusion efficiency.
利用基于十八烷基罗丹明（R18）荧光自猝灭消除的脂质混合检测法，研究了仙台病毒膜与脂质体或人红细胞血影之间的融合。我们只考虑反映病毒刺突糖蛋白生物活性的病毒融合。脂质体由磷脂酰胆碱制成，并测试了包含胆固醇、唾液酸糖脂GD1a和/或唾液酸糖蛋白血型糖蛋白作为受体的效果。仙台病毒在37℃下与这些脂质体的结合非常弱。与红细胞膜的融合速度比与脂质体的融合速度快30倍。对不同大小的生物和脂质体靶标的实验表明，大小不能解释融合效率的差异。
