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微生物转谷氨酰胺酶增强骆驼奶酸奶的物理化学、微观结构和流变学特性

Physico-chemical, microstructural and rheological properties of camel-milk yogurt as enhanced by microbial transglutaminase.

作者信息

Abou-Soliman Nagwa H I, Sakr Sally S, Awad Sameh

机构信息

Animal Breeding Department, Animal and Poultry Production Division, Desert Research Centre, Matariya, Cairo, Egypt.

Dairy Science Department, Faculty of Agriculture, Cairo University, 3 El-Gamaa Street, Giza, Egypt.

出版信息

J Food Sci Technol. 2017 May;54(6):1616-1627. doi: 10.1007/s13197-017-2593-9. Epub 2017 Mar 27.

Abstract

Camel milk produces watery texture when it is processed to yogurt. Despite the extensive studies on microbial transglutaminase (MTGase) in dairy research, the effect of this enzyme on the properties of yogurt made from camel milk has not been studied. This study aims to investigate the impact of MTGase with and without bovine skimmed milk powder (SMP), whey protein concentrate (WPC),or β-lactoglobulin (β-lg) on physico-chemical, rheological, microstructural, and sensory properties of camel-milk yogurt during 15 days of storage period. MTGase treatment markedly reduced the fermentation time of unfortified and SMP-fortified camel milk. The fortification of camel milk without MTGase failed to give set-type yogurt. The treatment of unfortified milk with MTGase enormously improved the viscosity and the body of yogurt samples. Fortification of MTGase-treated milk impacted positively on the viscosity, the water holding capacity, and the density of the protein matrix in the gel microstructure, which were influenced by the type of dairy ingredients. All MTGase-treated yogurts differed from each other in hardness and adhesiveness values. Electrophoresis results showed that the susceptibility of the individual milk proteins to MTGase varied, and there were differences among the treatments toward the enzyme. SMP-fortified yogurt was the most accepted product. Generally, the addition of MTGase preparation at a concentration of 0.4%, simultaneously with starter culture, to fortified camel milk was considered an effective tool to solve the challenges of producing set-type yogurt from this milk.

摘要

骆驼奶加工成酸奶后质地较稀。尽管在乳制品研究中对微生物转谷氨酰胺酶(MTGase)进行了广泛研究,但该酶对骆驼奶酸奶特性的影响尚未得到研究。本研究旨在探讨添加和不添加脱脂奶粉(SMP)、浓缩乳清蛋白(WPC)或β-乳球蛋白(β-lg)的MTGase对骆驼奶酸奶在储存15天期间的物理化学、流变学、微观结构和感官特性的影响。MTGase处理显著缩短了未强化和添加SMP的骆驼奶的发酵时间。未添加MTGase的骆驼奶强化处理未能制成凝固型酸奶。用MTGase处理未强化的牛奶极大地提高了酸奶样品的粘度和质地。MTGase处理的牛奶强化对凝胶微观结构中蛋白质基质的粘度、持水能力和密度有积极影响,这受到乳制品成分类型的影响。所有经MTGase处理的酸奶在硬度和粘附性值方面彼此不同。电泳结果表明,单个乳蛋白对MTGase的敏感性不同,各处理对该酶的反应也存在差异。添加SMP的酸奶是最受欢迎的产品。一般来说,在强化骆驼奶中,以0.4%的浓度同时添加MTGase制剂和发酵剂被认为是解决用这种牛奶生产凝固型酸奶难题的有效方法。

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