用于分析草莓树（Arbutus unedo L.）蜂蜜中尿黑酸的气相色谱 - 质谱联用（GC-MS）方法的开发与验证
Development and Validation of a GC-MS Method for the Analysis of Homogentisic Acid in Strawberry Tree ( L.) Honey.
作者信息
Brčić Karačonji Irena, Jurica Karlo
机构信息
Institute for Medical Research and Occupational Health, Analytical Toxicology and Mineral Metabolism Unit, Ksaverska cesta 2, 10001 Zagreb, Croatia.
Ministry of the Interior, Special Security Operations Directorate, Ulica grada Vukovara 33, 10000 Zagreb, Croatia.
出版信息
J AOAC Int. 2017 Jul 1;100(4):889-892. doi: 10.5740/jaoacint.17-0148. Epub 2017 May 31.
To confirm the botanical origin of strawberry tree (Arbutus unedo L.) honey, a liquid-liquid extraction followed by GC-MS method was developed for the quantitative determination of homogentisic acid (HGA), the main phenolic compound in this honey. Different parameters affecting extraction, such as the type and volume of extraction solvents, pH of the solution, and amount of salt, were optimized. The method showed good linearity (r2 = 0.9990) over the tested concentration range (50-500 mg/kg) and a low LOD (0.3 mg/kg). Precision expressed as RSD was <7%. The average accuracy was 95%. The optimized method was applied for determining the HGA content in strawberry tree honey samples from Croatia. The HGA content in analyzed samples (n = 7) ranged from 245.1 to 485.9 mg/kg. The proposed method provided reliable performance and can be easily implemented for the routine monitoring of HGA in strawberry tree honey in order to assure honey QC.
为了确定草莓树（Arbutus unedo L.）蜂蜜的植物来源，开发了一种液-液萃取结合气相色谱-质谱联用（GC-MS）的方法，用于定量测定该蜂蜜中的主要酚类化合物尿黑酸（HGA）。对影响萃取的不同参数进行了优化，如萃取溶剂的类型和体积、溶液的pH值以及盐的用量。该方法在测试浓度范围（50 - 500 mg/kg）内显示出良好的线性（r2 = 0.9990），检测限低（0.3 mg/kg）。以相对标准偏差（RSD）表示的精密度<7%。平均准确度为95%。将优化后的方法应用于测定克罗地亚草莓树蜂蜜样品中的HGA含量。分析样品（n = 7）中的HGA含量在245.1至485.9 mg/kg之间。所提出的方法性能可靠，可轻松用于草莓树蜂蜜中HGA的常规监测，以确保蜂蜜质量控制。
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