利用微型基因组系统对埃博拉病毒基因组复制和转录进行建模

Modeling Ebola Virus Genome Replication and Transcription with Minigenome Systems.

作者信息

Cressey Tessa, Brauburger Kristina, Mühlberger Elke

机构信息

Department of Microbiology and National Emerging Infectious Diseases Laboratories, Boston University, School of Medicine, Boston, MA, USA.

Department of Biology, Lund University, Lund, Sweden.

出版信息

Methods Mol Biol. 2017;1628:79-92. doi: 10.1007/978-1-4939-7116-9_6.

DOI:10.1007/978-1-4939-7116-9_6

PMID:28573612

Abstract

In this chapter, we describe the minigenome system for Ebola virus (EBOV), which reconstitutes EBOV polymerase activity in cells and can be used to model viral genome replication and transcription. This protocol comprises all steps including cell culture, plasmid preparation, transfection, and luciferase reporter assay readout.

摘要

在本章中，我们描述了埃博拉病毒（EBOV）的微型基因组系统，该系统可在细胞中重建EBOV聚合酶活性，并可用于模拟病毒基因组的复制和转录。本方案包括细胞培养、质粒制备、转染和荧光素酶报告基因检测读数等所有步骤。