用病毒样颗粒模拟埃博拉病毒出芽

Modeling Ebolavirus Budding with Virus Like Particles.

作者信息

Reynard Olivier, Mateo Mathieu

机构信息

INSERM U1111-CNRS UMR5308, Centre International de Recherche en Infectiologie (CIRI), Université Claude Bernard Lyon 1, Ecole Normale Supérieure de Lyon, Lyon, France.

UBIVE, Institut Pasteur, CIRI, Lyon, France.

出版信息

Methods Mol Biol. 2017;1628:109-117. doi: 10.1007/978-1-4939-7116-9_8.

DOI:10.1007/978-1-4939-7116-9_8

PMID:28573614

Abstract

About 15 years ago, several groups initially described the release of virus like particles (VLPs) upon expression of Ebola virus VP40 in mammalian cells. Further development of the protocol later allowed for the dissection of the Ebola virus budding mechanism and for the identification of critical VP40 residues involved in this process. VLPs are now produced routinely in several laboratories as a tool to study virus entry or egress and have even been proposed as vaccine candidates against Ebola virus disease. Here we described protocols for the production and the analysis of Ebola virus VLP release.

摘要

大约15年前，几个研究小组最初描述了在哺乳动物细胞中表达埃博拉病毒VP40时会释放病毒样颗粒（VLP）。该实验方案的进一步发展使得对埃博拉病毒出芽机制的剖析以及对参与这一过程的关键VP40残基的鉴定成为可能。如今，VLP在多个实验室中已常规生产，作为研究病毒进入或释放的工具，甚至有人提议将其作为抗埃博拉病毒病的候选疫苗。在此，我们描述了埃博拉病毒VLP释放的生产和分析方案。