Ma Xiaocui, Wang Guodong, Zhao Weiyang, Yang Minmin, Ma Nana, Kong Fanying, Dong Xinchun, Meng Qingwei
College of Life Science, State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, Shandong 271018, PR China.
College of Life Science, State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, Shandong 271018, PR China; College of Biological Science, Jining Medical University, Rizhao, Shandong 276800, PR China.
J Plant Physiol. 2017 Sep;216:88-99. doi: 10.1016/j.jplph.2017.03.016. Epub 2017 Mar 28.
Drought stress adversely affects plant growth, development, and productivity. Genes functioning in plant response to drought stress are essential for drought tolerance. In this study, SlCOR413IM1, a cold-regulated gene isolated from Solanum lycopersium, was transferred to Nicotiana tabacum to investigate its function under drought stress. The subcellular localisation of SlCOR413IM1-GFP fusion protein in Arabidopsis protoplasts suggested that SlCOR413IM1 is a chloroplast protein. Expression analyses revealed that SlCOR413IM1 responded to drought and cold stresses. Under drought stress, transgenic plants maintained the high maximum photochemical efficiency, net photosynthetic rate (Pn) and D1 protein content of photosystem II (PSII). Compared with wild-type (WT) plants, transgenic plants showed higher superoxide dismutase (SOD) and ascorbate peroxidase (APX) activities and proline and soluble sugar content, which reduced reactive oxygen species (ROS) generation. However, the high SOD and APX activities in transgenic plants were independent of their transcription levels. Moreover, the transgenic plants exhibited better seed germination, water status and survival, as well as lower malondialdehyde (MDA) content and relative electrical conductivity (REC) than WT plants under drought stress. Taken together, these data demonstrated that overexpression of SlCOR413IM1 enhanced drought stress tolerance in transgenic tobacco.
干旱胁迫对植物的生长、发育和生产力产生不利影响。在植物对干旱胁迫的响应中发挥作用的基因对于耐旱性至关重要。在本研究中，从番茄中分离出的一个冷调节基因SlCOR413IM1被转入烟草中，以研究其在干旱胁迫下的功能。SlCOR413IM1-GFP融合蛋白在拟南芥原生质体中的亚细胞定位表明SlCOR413IM1是一种叶绿体蛋白。表达分析显示SlCOR413IM1对干旱和冷胁迫有响应。在干旱胁迫下，转基因植物保持了较高的最大光化学效率、净光合速率（Pn）和光系统II（PSII）的D1蛋白含量。与野生型（WT）植物相比，转基因植物表现出更高的超氧化物歧化酶（SOD）和抗坏血酸过氧化物酶（APX）活性以及脯氨酸和可溶性糖含量，这减少了活性氧（ROS）的产生。然而，转基因植物中较高的SOD和APX活性与其转录水平无关。此外，在干旱胁迫下，转基因植物比WT植物表现出更好的种子萌发、水分状况和存活率，以及更低的丙二醛（MDA）含量和相对电导率（REC）。综上所述，这些数据表明SlCOR413IM1的过表达增强了转基因烟草对干旱胁迫的耐受性。
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