消化后锇的稳定化使得其能够通过 ICP-MS 在细胞培养和组织中进行定量分析。
Post-digestion stabilization of osmium enables quantification by ICP-MS in cell culture and tissue.
机构信息
Institute of Inorganic Chemistry, University of Vienna, Vienna, Austria.
出版信息
Analyst. 2017 Jun 26;142(13):2327-2332. doi: 10.1039/c7an00350a.
An orally active osmium anticancer compound was reliably quantified in the organs of treated mice by inductively coupled plasma-mass spectrometry (ICP-MS) by adding a stabilizing solution consisting of ascorbic acid, thiourea and EDTA during sample preparation and avoiding oxidizing conditions. The limits of detection (LOD) and quantification (LOQ) of Os were determined in liver tissue to be 0.02 and 0.075 μg kg, respectively. In spiked liver tissue, the internal precision showed a relative standard deviation (RSD) of 4%, a matrix recovery of 92% and a digestion recovery of 99%. A similar quantification protocol was developed for cellular accumulation studies in vitro. The cells were lysed with a non-oxidizing lysis buffer consisting of 150 mmol L NaCl, 1.0% Triton X-100, 0.1% SDS, and 50 mmol L Tris at pH 8.0 before adding the stabilizing solution. The osmium compound was compared with an isosteric ruthenium analogue and they displayed similar cellular accumulation and organ distribution profiles.
通过在样品制备过程中添加含有抗坏血酸、硫脲和 EDTA 的稳定溶液并避免氧化条件，用电感耦合等离子体质谱法（ICP-MS）可在经处理的小鼠的器官中可靠地定量分析具有口服活性的锇抗癌化合物。在肝组织中，确定了 Os 的检测限（LOD）和定量限（LOQ）分别为 0.02 和 0.075 μg kg。在加标肝组织中，内部精密度的相对标准偏差（RSD）为 4%，基质回收率为 92%，消化回收率为 99%。为体外细胞积累研究开发了类似的定量方案。在加入稳定溶液之前，用含有 150 mmol L NaCl、1.0% Triton X-100、0.1% SDS 和 50 mmol L Tris（pH 8.0）的非氧化裂解缓冲液裂解细胞。将锇化合物与等电子的钌类似物进行比较，它们显示出相似的细胞积累和器官分布特征。
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