Jordão Camila P, Fernandes Tiago, Tanaka Leonardo Yuji, Bechara Luiz R Grassmann, de Sousa Luis Gustavo Oliveira, Oliveira Edilamar M, Ramires Paulo Rizzo
Unidade de Reabilitação, Instituto do Coracao (InCor), Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo, SP, BR.
Laboratorio de Bioquimica e Biologia Molecular do Exercicio, Escola de Educacao Fisica e Esporte, Universidade de Sao Paulo, Sao Paulo, SP, BR.
Clinics (Sao Paulo). 2017 May;72(5):310-316. doi: 10.6061/clinics/2017(05)09.
: We aimed to determine whether aerobic training decreases superoxide levels, increases nitric oxide levels, and improves endothelium-dependent vasodilation in the aortas of spontaneously hypertensive rats.
: Spontaneously hypertensive rats (SHR) and Wistar Kyoto rats (WKY) were distributed into 2 groups: sedentary (SHRsd and WKYsd, n=10 each) and swimming-trained (SHRtr, n=10 and WKYtr, n=10, respectively). The trained group participated in training sessions 5 days/week for 1 h/day with an additional work load of 4% of the animal's body weight. After a 10-week sedentary or aerobic training period, the rats were euthanized. The thoracic aortas were removed to evaluate the vasodilator response to acetylcholine (10-10 to 10-4 M) with or without preincubation with L-NG-nitro-L-arginine methyl ester hydrochloride (L-NAME; 10-4 M) in vitro. The aortic tissue was also used to assess the levels of the endothelial nitric oxide synthase and nicotinamide adenine dinucleotide oxidase subunit isoforms 1 and 4 proteins, as well as the superoxide and nitrite contents. Blood pressure was measured using a computerized tail-cuff system.
: Aerobic training significantly increased the acetylcholine-induced maximum vasodilation observed in the SHRtr group compared with the SHRsd group (85.9±4.3 vs. 71.6±5.2%). Additionally, in the SHRtr group, superoxide levels were significantly decreased, nitric oxide bioavailability was improved, and the levels of the nicotinamide adenine dinucleotide oxidase subunit isoform 4 protein were decreased compared to the SHRsd group. Moreover, after training, the blood pressure of the SHRtr group decreased compared to the SHRsd group. Exercise training had no effect on the blood pressure of the WKYtr group.
: In SHR, aerobic swim training decreased vascular superoxide generation by nicotinamide adenine dinucleotide oxidase subunit isoform 4 and increased nitric oxide bioavailability, thereby improving endothelial function.
我们旨在确定有氧训练是否能降低自发性高血压大鼠主动脉中的超氧化物水平、增加一氧化氮水平并改善内皮依赖性血管舒张功能。
将自发性高血压大鼠（SHR）和Wistar Kyoto大鼠（WKY）分为两组：久坐组（SHRsd和WKYsd，每组n = 10）和游泳训练组（SHRtr，n = 10；WKYtr，n = 10）。训练组每周进行5天、每天1小时的训练课程，并额外增加相当于动物体重4%的工作量。经过10周的久坐或有氧训练期后，将大鼠安乐死。取出胸主动脉，在体外评估其对乙酰胆碱（10⁻¹⁰至10⁻⁴M）的血管舒张反应，以及在有无预先用盐酸L-NG-硝基-L-精氨酸甲酯（L-NAME；10⁻⁴M）孵育的情况下的反应。主动脉组织还用于评估内皮型一氧化氮合酶、烟酰胺腺嘌呤二核苷酸氧化酶亚基同工型1和4蛋白的水平，以及超氧化物和亚硝酸盐含量。使用计算机化尾袖带系统测量血压。
与SHRsd组相比，有氧训练显著增加了SHRtr组中观察到的乙酰胆碱诱导的最大血管舒张（85.9±4.3%对71.6±5.2%）。此外，与SHRsd组相比，SHRtr组中的超氧化物水平显著降低，一氧化氮生物利用度得到改善，烟酰胺腺嘌呤二核苷酸氧化酶亚基同工型4蛋白的水平降低。此外，训练后，SHRtr组的血压与SHRsd组相比有所下降。运动训练对WKYtr组的血压没有影响。
在SHR中，有氧游泳训练可减少烟酰胺腺嘌呤二核苷酸氧化酶亚基同工型4产生的血管超氧化物，并增加一氧化氮生物利用度，从而改善内皮功能。
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