Gupta Swati, Pandey Sriti, Parmar Mehtab S, Somal Anjali, Paul Avishek, Panda Bibhudatta S K, Bhat Irfan A, Baiju Indu, Bharti Mukesh K, Saikumar G, Sarkar Mihir, Chandra Vikash, Sharma G Taru
Reproductive Physiology Laboratory,Division of Physiology and Climatology,ICAR-Indian Veterinary Research Institute,Izatnagar-243 122,Bareilly,UP,India.
Zygote. 2017 Jun;25(3):313-320. doi: 10.1017/S0967199417000156. Epub 2017 Jun 8.
Oocyte-secreted factors (OSFs) play an important role in the acquisition of oocyte developmental competence through bidirectional cross-talk between oocyte and cumulus cells via gap junctions. Thus, the present study was designed to investigate the effect of two OSFs, growth differentiation factor 9 (GDF9) and bone morphogenetic protein 15 (BMP15), on the developmental competence of buffalo oocytes derived from two different follicle sizes. Cumulus-oocyte complexes (COCs) from large follicles (LF, >6 mm) or small follicles (SF, 0.05) between DOs and combination groups. Relative mRNA analysis revealed significantly higher (P > 0.05) expression of the cumulus cell marker genes EGFR, HAS2, and CD44 in LF-derived than SF-derived oocyte; the expression of these markers was significantly higher (P > 0.05) in DOs and combination groups, irrespective of the follicle size. These results suggested that LF-derived oocytes have a higher developmental competence than SF-derived oocytes and that supplementation of GDF9 and BMP15 modulates the developmental competence of buffalo oocytes by increasing the relative abundance of cumulus-enabling factors and thereby increasing cleavage and the quality of blastocyst production.
卵母细胞分泌因子（OSFs）通过间隙连接在卵母细胞与卵丘细胞之间的双向串扰，在卵母细胞发育能力的获得中发挥重要作用。因此，本研究旨在探讨两种卵母细胞分泌因子，即生长分化因子9（GDF9）和骨形态发生蛋白15（BMP15），对源自两种不同卵泡大小的水牛卵母细胞发育能力的影响。从大卵泡（LF，>6毫米）或小卵泡（SF，<3毫米）收集卵丘-卵母细胞复合体（COCs）。将其随机分为四组：对照组（未处理）、GDF9处理组（DOs）、BMP15处理组和联合处理组（GDF9+BMP15）。体外成熟培养后，评估卵母细胞的成熟率、卵裂率和囊胚发育率。结果显示，与小卵泡来源的卵母细胞相比，大卵泡来源的卵母细胞在成熟率、卵裂率和囊胚发育率方面均显著更高（P<0.05）。在成熟率方面，联合处理组显著高于对照组（P<0.05），但与单独处理组相比无显著差异（P>0.05）。在卵裂率和囊胚发育率方面，联合处理组显著高于对照组和单独处理组（P<0.05）。相对mRNA分析显示，大卵泡来源的卵母细胞中卵丘细胞标记基因EGFR、HAS2和CD44的表达显著高于小卵泡来源的卵母细胞（P>0.05）；无论卵泡大小，这些标记物在单独处理组和联合处理组中的表达均显著更高（P>0.05）。这些结果表明，大卵泡来源的卵母细胞比小卵泡来源的卵母细胞具有更高的发育能力，补充GDF9和BMP15可通过增加卵丘支持因子的相对丰度来调节水牛卵母细胞的发育能力，从而提高卵裂率和囊胚产生的质量。
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