King A E, Nistri A, Rovira C
Neurosci Lett. 1985 Mar 22;55(1):77-82. doi: 10.1016/0304-3940(85)90315-5.
Intracellular recordings were made from motoneurones of the frog spinal cord in vitro and the excitatory effects of the glutamate analogue DL-alpha-amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid (AMPA) examined. AMPA (25-100 microM) depolarized motoneurones with an increase in input conductance and an increase in spontaneous cell firing. The time-course of the AMPA response was prolonged compared to L-glutamate. D-Aminophosphonovalerate (1 microM) and D-aminoadipate (100 microM) did not antagonize AMPA-induced excitations while depressing L-glutamate responses. Glutamate diethylester up to 1 mM was not found to be a useful amino acid antagonist since it did not significantly reduce the depolarizations to quisqualate, L-glutamate and AMPA. The results are compatible with an action of AMPA on receptors distinct from those to N-methyl-D-aspartate and shared by quisqualate.
在体外对青蛙脊髓运动神经元进行细胞内记录，并检测了谷氨酸类似物DL-α-氨基-3-羟基-5-甲基-4-异恶唑丙酸（AMPA）的兴奋作用。AMPA（25 - 100微摩尔）使运动神经元去极化，同时输入电导增加，细胞自发放电增多。与L-谷氨酸相比，AMPA反应的时程延长。D-氨基磷酸戊酸（1微摩尔）和D-氨基己二酸（100微摩尔）在抑制L-谷氨酸反应的同时，并不拮抗AMPA诱导的兴奋。高达1毫摩尔的谷氨酸二乙酯未被发现是一种有效的氨基酸拮抗剂，因为它并未显著降低对喹啉酸、L-谷氨酸和AMPA的去极化作用。这些结果与AMPA作用于不同于N-甲基-D-天冬氨酸受体且与喹啉酸共有的受体相一致。
