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BioInfra.Prot:一个全面的蛋白质组学工作流程,包括数据标准化、蛋白质推断、表达分析和数据发布。

BioInfra.Prot: A comprehensive proteomics workflow including data standardization, protein inference, expression analysis and data publication.

机构信息

Medizinisches Proteom-Center, Ruhr-University Bochum, Bochum, Germany.

Medizinisches Proteom-Center, Ruhr-University Bochum, Bochum, Germany; Institute for Virology, University Hospital Essen, University Duisburg-Essen, Essen, Germany.

出版信息

J Biotechnol. 2017 Nov 10;261:116-125. doi: 10.1016/j.jbiotec.2017.06.005. Epub 2017 Jun 9.

Abstract

The analysis of high-throughput mass spectrometry-based proteomics data must address the specific challenges of this technology. To this end, the comprehensive proteomics workflow offered by the de.NBI service center BioInfra.Prot provides indispensable components for the computational and statistical analysis of this kind of data. These components include tools and methods for spectrum identification and protein inference, protein quantification, expression analysis as well as data standardization and data publication. All particular methods of the workflow which address these tasks are state-of-the-art or cutting edge. As has been shown in previous publications, each of these methods is adequate to solve its specific task and gives competitive results. However, the methods included in the workflow are continuously reviewed, updated and improved to adapt to new scientific developments. All of these particular components and methods are available as stand-alone BioInfra.Prot services or as a complete workflow. Since BioInfra.Prot provides manifold fast communication channels to get access to all components of the workflow (e.g., via the BioInfra.Prot ticket system: bioinfraprot@rub.de) users can easily benefit from this service and get support by experts.

摘要

基于高通量质谱的蛋白质组学数据分析必须解决该技术的特定挑战。为此,de.NBI 服务中心 BioInfra.Prot 提供的综合蛋白质组学工作流程为这种数据的计算和统计分析提供了不可或缺的组成部分。这些组成部分包括用于谱识别和蛋白质推断、蛋白质定量、表达分析以及数据标准化和数据发布的工具和方法。工作流程中用于解决这些任务的所有特定方法都是最先进的或最前沿的。如先前的出版物所示,这些方法中的每一种都足以解决其特定任务,并给出具有竞争力的结果。然而,工作流程中包含的方法会不断进行审查、更新和改进,以适应新的科学发展。所有这些特定的组件和方法都可以作为独立的 BioInfra.Prot 服务或作为一个完整的工作流程使用。由于 BioInfra.Prot 提供了多种快速通信渠道来访问工作流程的所有组件(例如,通过 BioInfra.Prot 票务系统:bioinfraprot@rub.de),用户可以轻松受益于这项服务,并得到专家的支持。

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