人外周血单个核细胞中lncRNA与mRNA整合模块的鉴定与评估
Identification and evaluation of lncRNA and mRNA integrative modules in human peripheral blood mononuclear cells.
作者信息
Mo Xing-Bo, Wu Long-Fei, Zhu Xiao-Wei, Xia Wei, Wang Lan, He Pei, Bing Peng-Fei, Lu Xin, Zhang Yong-Hong, Deng Fei-Yan, Lei Shu-Feng
机构信息
Center for Genetic Epidemiology & Genomics, School of Public Health, Medical College of Soochow University, Suzhou, Jiangsu 215123, PR China.
Jiangsu Key Laboratory of Preventive & Translational Medicine for Geriatric Diseases, Soochow University, Suzhou, Jiangsu 215123, PR China.
出版信息
Epigenomics. 2017 Jul;9(7):943-954. doi: 10.2217/epi-2016-0178. Epub 2017 Jun 16.
AIM
To identify sets of functionally related long noncoding RNAs (lncRNAs) and mRNAs and to evaluate the importance of lncRNAs in an lncRNA-mRNA network.
METHODS
We carried out weighted gene co-expression network analysis and enrichment analyses to identify functional modules of co-expressed lncRNAs and mRNAs in human peripheral blood mononuclear cells of 43 females.
RESULTS
We identified seven modules and found hub lncRNAs in each module. Four of the seven modules had significant gene ontology enrichments. Some of the hub lncRNAs (e.g., SSX8, UCA1, HOXA-AS2, STARD4-AS1 and PCBP1-AS1) have known functions related with diseases such as cancers.
CONCLUSION
We identified seven biologically important lncRNA and mRNA integrative modules in females and showed that lncRNAs might play important roles in lncRNA-mRNA co-expression modules.
目的
鉴定功能相关的长链非编码RNA（lncRNA）和信使核糖核酸（mRNA）集合，并评估lncRNA在lncRNA-mRNA网络中的重要性。
方法
我们进行了加权基因共表达网络分析和富集分析，以鉴定43名女性人外周血单个核细胞中共表达的lncRNA和mRNA的功能模块。
结果
我们鉴定出7个模块，并在每个模块中发现了枢纽lncRNA。7个模块中的4个具有显著的基因本体富集。一些枢纽lncRNA（例如，SSX8、UCA1、HOXA-AS2、STARD4-AS1和PCBP1-AS1）具有与癌症等疾病相关的已知功能。
结论
我们在女性中鉴定出7个具有生物学重要性的lncRNA和mRNA整合模块，并表明lncRNA可能在lncRNA-mRNA共表达模块中发挥重要作用。
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