组织工程化骨膜修复兔同种异体临界尺寸骨缺损的体内研究
In vivo investigation of tissue-engineered periosteum for the repair of allogeneic critical size bone defects in rabbits.
作者信息
Zhao Lin, Zhao Junli, Yu Jiajia, Sun Rui, Zhang Xiaofeng, Hu Shuhua
机构信息
Orthopaedic Department, Jinshan Branch of the Sixth People's Hospital of Shanghai, Shanghai Jiao Tong University, Shanghai 201500, China.
Department of Nephrology, Shanghai ZhouPu Hospital, Shanghai 201318, China.
出版信息
Regen Med. 2017 Apr;12(4):353-364. doi: 10.2217/rme-2016-0157. Epub 2017 Jun 16.
AIM
The aim of the study was to evaluate the efficacy of tissue-engineered periosteum (TEP) in repairing allogenic bone defects in the long term.
MATERIALS & METHODS: TEP was biofabricated with osteoinduced rabbit bone marrow mesenchymal stem cells and porcine small intestinal submucosa (SIS). A total of 24 critical sized defects were created bilaterally in radii of 12 New Zealand White rabbits. TEP/SIS was implanted into the defect site. Bone defect repair was evaluated with radiographic and histological examination at 4, 8 and 12 weeks.
RESULTS
Bone defects were structurally reconstructed in the TEP group with mature cortical bone and medullary canals, however this was not observed in the SIS group at 12 weeks.
CONCLUSION
The TEP approach can effectively restore allogenic critical sized defects, and achieve maturity of long-bone structure in 12 weeks in rabbit models.
目的
本研究旨在长期评估组织工程骨膜（TEP）修复同种异体骨缺损的疗效。
材料与方法
用诱导成骨的兔骨髓间充质干细胞和猪小肠黏膜下层（SIS）生物制造TEP。在12只新西兰白兔的双侧桡骨上制造了总共24个临界尺寸缺损。将TEP/SIS植入缺损部位。在4、8和12周时通过影像学和组织学检查评估骨缺损修复情况。
结果
TEP组骨缺损通过成熟的皮质骨和髓腔进行了结构重建，然而在12周时SIS组未观察到这种情况。
结论
在兔模型中，TEP方法可有效修复同种异体临界尺寸缺损，并在12周内实现长骨结构成熟。
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