Arslan Ibrahim, Keles Erol, Ozercan I Hanifi, Tokdemir Mehmet, Karlidag Turgut, Kaygusuz Irfan, Eroglu Orkun, Yuksel Koray, Yalcin Sinasi
Department of Otorhinolaryngology, School of Medicine, Fırat University, Elazig, Turkey.
Department of Pathology, School of Medicine, Fırat University, Elazig, Turkey.
Eur Arch Otorhinolaryngol. 2017 Sep;274(9):3443-3447. doi: 10.1007/s00405-017-4644-2. Epub 2017 Jun 17.
Pleomorphic adenoma is a slow-growing salivary gland tumor frequently arising from the parotid gland. In this study, we investigated the role of the insulin-like growth factor I-II receptor (IGFI-IIR) levels on the development of parotid gland pleomorphic adenomas. The study included 20 males and 20 females who had superficial parotidectomy with a histopathological diagnosis of pleomorphic adenoma in Fırat University Otorhinolaryngology Clinic between 2000 and 2011. The ages of the patients ranged between 20 and 50 years. The control tissues were obtained unilaterally from the parotid glands of five female and five male cadavers during autopsy, and consisted of 0.5 × 0.5 cm sized normal parotid gland tissues. The expression of IGFI-IIR were measured in both tumor and tumor-free normal parotid tissue in the study group while only the normal parotid tissues were studied in the cadavers. Primary polyclonal antibodies against IGFI-IIR were used with "Streptavidin-Biotin Complex" method for immunohistochemical staining of both the study and the control groups' tissue sections. In this study, the IGFI-IIR levels were found significantly higher in the pleomorphic adenoma tissue (p < 0.05). In addition, IGFI-IIR expression was greater in normal parotid tissues of the study group when compared to the normal parotid tissues of the cadavers. However, the difference was not statistically significant (p > 0.017). Greater expression for IGFI-IIR in pleomorphic adenoma when compared to normal parotid tissues of the patients and the cadavers suggests that IGFI-II may be important factors in the development of pleomorphic adenoma.
多形性腺瘤是一种生长缓慢的唾液腺肿瘤，常起源于腮腺。在本研究中，我们调查了胰岛素样生长因子I-II受体（IGFI-IIR）水平在腮腺多形性腺瘤发生发展中的作用。该研究纳入了2000年至2011年间在菲拉特大学耳鼻喉科诊所接受腮腺浅叶切除术且组织病理学诊断为多形性腺瘤的20名男性和20名女性。患者年龄在20至50岁之间。对照组织是在尸检时从5名男性和5名女性尸体的腮腺单侧获取的，由大小为0.5×0.5 cm的正常腮腺组织组成。研究组同时检测了肿瘤组织和无肿瘤的正常腮腺组织中IGFI-IIR的表达，而在尸体中仅研究了正常腮腺组织。针对IGFI-IIR的一抗采用“链霉亲和素-生物素复合物”方法对研究组和对照组的组织切片进行免疫组织化学染色。在本研究中，发现多形性腺瘤组织中IGFI-IIR水平显著更高（p<0.05）。此外，与尸体的正常腮腺组织相比，研究组正常腮腺组织中IGFI-IIR的表达更高。然而，差异无统计学意义（p>0.017）。与患者和尸体的正常腮腺组织相比，多形性腺瘤中IGFI-IIR表达更高，提示IGFI-II可能是多形性腺瘤发生发展中的重要因素。
