Brown D L, Little J E, Chaly N, Schweitzer I, Paulin-Levasseur M
Eur J Cell Biol. 1985 May;37:130-9.
We have used immunofluorescence staining with antibodies to tubulin and electron microscopy to examine the effects of microtubule assembly-promoting drug taxol on the organization of microtubules in unstimulated and in mitogen stimulated mouse splenic lymphocytes. A 4-h exposure to 10 microM taxol of unstimulated or stimulated cells results in an extensive reorganization of the microtubule system to form one to a few large bundles of microtubules which extend from the centrosome. Concomitantly, the centrosome is displaced from the normal position near the nucleus to a position near the plasma membrane. The Golgi apparatus is not disrupted by this treatment and also appears to be displaced in association with the centrosome. We then examined the capacity of lymphocytes with taxol-reorganized microtubules to respond to stimulation by the mitogen concanavalin A. The taxol-induced reorganization of microtubules has no effect on the increase in cell size (blastogenesis), the changes in structure of the nucleus and cytoplasm, or on the DNA replication that occurs in response to mitogen. The stimulated cells, however, do not proliferate and appear to accumulate in mitosis with the appearance of multiple asters. We conclude that all of the major events of mitogenic stimulation up to the first mitosis can occur in the presence of a highly reorganized microtubule system. Our results suggest that taxol will be a useful drug in determining those lymphocyte functions that are dependent on the presence of normal microtubule organization.
我们运用针对微管蛋白的抗体进行免疫荧光染色以及电子显微镜技术，来检测促进微管组装的药物紫杉醇对未受刺激和有丝分裂原刺激的小鼠脾淋巴细胞中微管组织的影响。将未受刺激或受刺激的细胞暴露于10微摩尔的紫杉醇中4小时，会导致微管系统发生广泛重组，形成从中心体延伸出的一到几束大的微管束。与此同时，中心体从靠近细胞核的正常位置移位到靠近质膜的位置。高尔基体并未因这种处理而受到破坏，并且似乎也随着中心体一起移位。然后我们检测了具有紫杉醇重组微管的淋巴细胞对有丝分裂原伴刀豆球蛋白A刺激的反应能力。紫杉醇诱导的微管重组对细胞大小增加（细胞增殖）、细胞核和细胞质结构的变化或对有丝分裂原反应时发生的DNA复制均无影响。然而，受刺激的细胞不增殖，似乎在有丝分裂中积累并出现多个星体。我们得出结论，在高度重组的微管系统存在的情况下，有丝分裂原刺激直至第一次有丝分裂的所有主要事件都可以发生。我们的结果表明，紫杉醇将是一种有用的药物，可用于确定那些依赖于正常微管组织存在的淋巴细胞功能。
