埃及人群中丙型肝炎病毒载量与丙型肝炎核心抗原血症之间的相关性。

Correlation between hepatitis C viral load and hepatitis C Core antigenaemia among Egyptians.

作者信息

Kotb Dalia N, Esmail Mona A, Abdelwahab Sayed F, Abdel-Hamid Mohamed

机构信息

Department of Microbiology and Immunology, Faculty of Medicine, Minia University, Minia, Egypt.

Department of Microbiology and Immunology, Faculty of Medicine, Minia University, Minia, Egypt; Department of Microbiology, Faculty of Pharmacy, Taif University, Al-Haweiah, Taif, Saudi Arabia.

出版信息

East Mediterr Health J. 2017 Jun 14;23(4):280-286. doi: 10.26719/2017.23.4.280.

DOI:10.26719/2017.23.4.280

PMID:28634978

Abstract

Hepatitis C virus (HCV) infection is widespread in Egypt. This study compared HCV RNA with HCVcAg for the detection and quantification of viraemia among a sample of Egyptians. Sera from 80 suspected HCVpositive individuals were tested simultaneously for HCV-RNA load using real-time polymerase chain reaction (PCR) and HCVcAg level using ELISA. Of the 80 samples, 25% were HCV-RNA-negative. HCVcAg was detected in all samples: range 0.4-2462 ng/mL, mean 460 (SD 506) ng/mL. The sensitivity and specificity of HCVcAg were 96.7% and 90.9%, respectively. There was a significant correlation between serum HCV-RNA and HCVcAg levels (r = 0.4, P < 0.0001). HCV-RNA remains the gold standard for diagnosis of active HCV infection but HCVcAg can be used where PCR is not available.

摘要

丙型肝炎病毒（HCV）感染在埃及广泛存在。本研究比较了HCV RNA与HCVcAg在埃及人群样本中检测和定量病毒血症的情况。使用实时聚合酶链反应（PCR）对80名疑似HCV阳性个体的血清同时检测HCV-RNA载量，并使用酶联免疫吸附测定（ELISA）检测HCVcAg水平。在这80个样本中，25%为HCV-RNA阴性。所有样本均检测到HCVcAg：范围为0.4 - 2462 ng/mL，平均为460（标准差506）ng/mL。HCVcAg的敏感性和特异性分别为96.7%和90.9%。血清HCV-RNA与HCVcAg水平之间存在显著相关性（r = 0.4，P < 0.0001）。HCV-RNA仍然是诊断活动性HCV感染的金标准，但在无法进行PCR检测的情况下，可以使用HCVcAg。