Department of Bionanotechnology, Hanyang University, Ansan, 155-88, South Korea.
Center of Neuroscience, University of Copenhagen, Copenhagen, DK-2200, Denmark.
Sci Rep. 2017 Jun 21;7(1):3963. doi: 10.1038/s41598-017-03809-3.
High-resolution membrane protein structures are essential for understanding the molecular basis of diverse biological events and important in drug development. Detergents are usually used to extract these bio-macromolecules from the membranes and maintain them in a soluble and stable state in aqueous solutions for downstream characterization. However, many eukaryotic membrane proteins solubilized in conventional detergents tend to undergo structural degradation, necessitating the development of new amphiphilic agents with enhanced properties. In this study, we designed and synthesized a novel class of glucoside amphiphiles, designated tandem malonate-based glucosides (TMGs). A few TMG agents proved effective at both stabilizing a range of membrane proteins and extracting proteins from the membrane environment. These favourable characteristics, along with synthetic convenience, indicate that these agents have potential in membrane protein research.
高分辨率膜蛋白结构对于理解各种生物事件的分子基础至关重要，并且在药物开发中也很重要。去污剂通常用于从膜中提取这些生物大分子，并在水溶液中保持其可溶性和稳定性，以进行下游表征。然而，许多在常规去污剂中溶解的真核膜蛋白容易发生结构降解，因此需要开发具有增强性能的新型两亲性试剂。在这项研究中，我们设计并合成了一类新型的糖苷两亲物，命名为基于丙二酸酯的糖苷（TMG）。一些 TMG 试剂被证明既能有效地稳定一系列膜蛋白，又能从膜环境中提取蛋白质。这些有利的特性，加上合成的便利性，表明这些试剂在膜蛋白研究中有潜力。
