Section of Experimental Oncology and Nanomedicine (SEON), Else Kröner-Fresenius-Stiftung-endowed Professorship for Nanomedicine, ENT Department, University Hospital Erlangen, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, Germany.
Department of Cardiology and Angiology, University Hospital Erlangen, Erlangen, Germany.
J Biomed Mater Res A. 2017 Nov;105(11):2948-2957. doi: 10.1002/jbm.a.36147. Epub 2017 Jul 14.
Tissue-engineered scaffolds require an effective colonization with cells. Superparamagnetic iron oxide nanoparticles (SPIONs) can enhance cell adhesion to matrices by magnetic cell seeding. We investigated the possibility of improving cell attachment and growth on different alginate-based hydrogels using fibroblasts and endothelial cells (ECs) loaded with SPIONs. Hydrogels containing pure alginate (Alg), alginate dialdehyde crosslinked with gelatin (ADA-G) and Alg blended with G or silk fibroin (SF) were prepared. Endothelial cells and fibroblasts loaded with SPIONs were seeded and grown on hydrogels for up to 7 days, in the presence of magnetic field during the first 24 h. Cell morphology (fluorescent staining) and metabolic activity (WST-8 assay) of magnetically-seeded versus conventionally seeded cells were compared. Magnetic seeding of ECs improved their initial attachment and further growth on Alg/G hydrogel surfaces. However, we did not achieve an efficient and stable colonization of ADA-G films with ECs even with magnetic cell seeding. Fibroblast showed good initial colonization and growth on ADA-G and on Alg/SF. This effect was further significantly enhanced by magnetic cell seeding. On pure Alg, initial attachment and spreading of magnetically-seeded cells was dramatically improved compared to conventionally-seeded cells, but the effect was transient and diminished gradually with the cessation of magnetic force. Our results demonstrate that magnetic seeding improves the strength and uniformity of initial cell attachment to hydrogel surface in cell-specific manner, which may play a decisive role for the outcome in tissue engineering applications. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 2948-2956, 2017.
组织工程支架需要有效地被细胞浸润。超顺磁氧化铁纳米颗粒（SPIONs）可以通过磁细胞接种增强细胞对基质的黏附。我们研究了用负载 SPIONs 的成纤维细胞和内皮细胞（ECs）来提高不同海藻酸钠基水凝胶的细胞黏附及生长的可能性。我们制备了含有纯海藻酸钠（Alg）、用明胶（ADA-G）交联的海藻酸钠二醛以及与明胶或丝素混合的海藻酸钠的水凝胶。将负载 SPIONs 的内皮细胞和成纤维细胞接种在水凝胶上，在磁场存在的情况下培养长达 7 天，在最初的 24 小时内进行磁接种。比较了磁性接种细胞与常规接种细胞的细胞形态（荧光染色）和代谢活性（WST-8 检测）。磁性接种 ECs 提高了它们在 Alg/G 水凝胶表面的初始黏附及进一步生长。然而，即使进行磁细胞接种，我们也未能使 ECs 有效地稳定定植在 ADA-G 薄膜上。成纤维细胞在 ADA-G 和 Alg/SF 上具有良好的初始定植和生长。这种效果通过磁细胞接种进一步显著增强。与常规接种细胞相比，负载 SPIONs 的细胞在纯 Alg 上的初始黏附及扩展得到了显著改善，但这种效果是短暂的，随着磁场的停止而逐渐减弱。我们的结果表明，磁接种以细胞特异性的方式改善了初始细胞对水凝胶表面的黏附强度和均匀性，这可能对组织工程应用的结果起决定性作用。© 2017 Wiley Periodicals, Inc. J 生物材料 Res 部分 A：105A：2948-2956，2017。
