Hwang Sungho, Ko Yeji, Park Donguk, Yoon Chungsik
National Cancer Control Institute, National Cancer Center, Ilsan, Korea.
Korea Workers' Compensation & Welfare Service, Seoul, Korea.
J Clin Pathol. 2018 Jan;71(1):59-66. doi: 10.1136/jclinpath-2017-204377. Epub 2017 Jun 30.
The purpose of this study was to assess the temporal changes in the concentrations of bioaerosols in a laboratory mouse room (LMR) and laboratory rabbit room (LRR), and to determine environmental factors associated with the culturable bacteria, fungi and (1→3)-β-D-glucan concentrations.
The concentrations of culturable airborne bacteria, fungi and (1→3)-β-D-glucan in the LMR and LRR were sampled once a month from March 2011 to February 2012. A single-stage viable cascade impactor was used to sample bacteria and fungi, while a two-stage cyclone bioaerosol sampler was used to collect airborne (1→3)-β-D-glucan.
The culturable bacterial concentrations in the LMR showed a gradual increase during the summer. The culturable fungal concentrations showed similar seasonal patterns of change in the LMR and LRR with a noticeable increase during the summer. The (1→3)-β-D-glucan concentrations were highest during the warmer spring and summer months. Relative humidity (RH) was the environmental factor most associated with the concentrations of culturable bacteria and fungi. The overall airborne microbe concentrations were significantly higher in the LRR than in the LMR.
Airborne microbe concentrations in the LMR and LRR varied greatly depending on season, and these changes were affected by environmental factors.
本研究旨在评估实验室小鼠房（LMR）和实验室兔房（LRR）中生物气溶胶浓度的时间变化，并确定与可培养细菌、真菌及（1→3）-β-D-葡聚糖浓度相关的环境因素。
2011年3月至2012年2月期间，每月对LMR和LRR中的可培养空气传播细菌、真菌及（1→3）-β-D-葡聚糖浓度进行一次采样。使用单级可行级联撞击器对细菌和真菌进行采样，同时使用两级旋风生物气溶胶采样器收集空气中的（1→3）-β-D-葡聚糖。
LMR中可培养细菌浓度在夏季呈逐渐上升趋势。LMR和LRR中可培养真菌浓度呈现相似的季节性变化模式，在夏季显著增加。（1→3）-β-D-葡聚糖浓度在较温暖的春季和夏季月份最高。相对湿度（RH）是与可培养细菌和真菌浓度最相关的环境因素。LRR中的总体空气微生物浓度显著高于LMR。
LMR和LRR中的空气微生物浓度随季节变化差异很大，且这些变化受环境因素影响。
