细胞表面暴露

Cell Surface Exposure.

作者信息

Konovalova Anna

机构信息

Lewis Thomas Laboratory, Department of Molecular Biology, Princeton University, Washington Road, Princeton, NJ, 08544, USA.

出版信息

Methods Mol Biol. 2017;1615:87-95. doi: 10.1007/978-1-4939-7033-9_7.

DOI:10.1007/978-1-4939-7033-9_7

PMID:28667605

Abstract

Surface-exposed proteins of Gram-negative bacteria are represented by integral outer membrane beta-barrel proteins and lipoproteins. No computational methods exist for predicting surface-exposed lipoproteins, and therefore lipoprotein topology must be experimentally tested. This chapter describes three distinct but complementary methods for the detection of surface-exposed proteins: cell surface protein labeling, accessibility to extracellular protease and antibodies.

摘要

革兰氏阴性菌的表面暴露蛋白由完整的外膜β-桶蛋白和脂蛋白组成。目前尚无预测表面暴露脂蛋白的计算方法，因此脂蛋白拓扑结构必须通过实验进行测试。本章介绍了三种不同但互补的检测表面暴露蛋白的方法：细胞表面蛋白标记、细胞外蛋白酶可及性和抗体检测。