两性霉素B增强阿霉素对MCF-7乳腺癌细胞的抗癌活性。
Amphotericin B potentiates the anticancer activity of doxorubicin on the MCF-7 breast cancer cells.
作者信息
Tavangar Farzaneh, Sepehri Hamid, Saghaeian Jazi Marie, Asadi Jahanbakhsh
机构信息
Student Research Committee, Golestan University of Medical Sciences, Gorgan, Iran.
Neuroscience Research Center, Golestan University of Medical Sciences, Gorgan, Iran.
出版信息
J Chem Biol. 2017 Jun 5;10(3):143-150. doi: 10.1007/s12154-017-0172-1. eCollection 2017 Jul.
Despite the improvements in cancer treatment, breast cancer still remains the second most common cause of death from cancer in women. Doxorubicin (DOXO) is widely used for cancer treatment. However, drug resistance limits the treatment outcome. Here, we investigated the toxicity of DOXO in combination with an antifungal agent amphotericin B (AmB) against the MCF-7 breast cancer cell line. The cell viability was measured using MTT assay. The apoptosis was studied by caspase-8 and caspase-9 activity measurements and DNA fragmentation was investigated by TUNEL assay. The combination of two drugs significantly increased the apoptotic index and the caspase-8 and caspase-9 activities in comparison to DOXO-treated cells. Our finding showed that pre-treatment of MCF-7 cells with AmB synergistically exerted the anticancer effect of DOXO through the caspase-dependent apoptosis manner.
尽管癌症治疗有所改善，但乳腺癌仍是女性癌症死亡的第二大常见原因。阿霉素（DOXO）广泛用于癌症治疗。然而，耐药性限制了治疗效果。在此，我们研究了DOXO与抗真菌剂两性霉素B（AmB）联合对MCF-7乳腺癌细胞系的毒性。使用MTT法测量细胞活力。通过测量caspase-8和caspase-9活性研究细胞凋亡，并通过TUNEL法研究DNA片段化。与DOXO处理的细胞相比，两种药物的联合显著增加了凋亡指数以及caspase-8和caspase-9活性。我们的研究结果表明，用AmB预处理MCF-7细胞通过caspase依赖性凋亡方式协同发挥DOXO的抗癌作用。
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