Iwata Tomoya, Nagatani Hirohisa, Osakai Toshiyuki
Department of Chemistry, Graduate School of Science, Kobe University.
Faculty of Chemistry, Institute of Science and Engineering, Kanazawa University.
Anal Sci. 2017;33(7):813-819. doi: 10.2116/analsci.33.813.
The fluorescence behaviors of potential-sensitive dyes including anionic DiBAC(3) (denoted by dye A), DiSBAC(3) (dye B), and zwitterionic di-4-ANEPPS (dye C) were studied in oil-in-water (O/W) emulsions. In this study, the equilibrium Galvani potential difference (Δφ) of the O/W-emulsion droplets was controlled by changing the ratio of the concentrations of electrolytes added to the O (=1,2-dichloroethane) and W phases. When using an adequate combination of the dyes, i.e., B and C, we could observe that the ratio of their fluorescence peak intensities was changed from 1.08 to 1.38, depending on the change of (Δφ from 26 to 73 mV. It is desirable to apply this method to study the potential-dependent ion or electron-transfer reactions occurring at vesicles or liposomes, and also to biomembranes.
研究了包括阴离子型DiBAC(3)（染料A）、DiSBAC(3)（染料B）和两性离子型二-4-ANEPPS（染料C）在内的电位敏感染料在水包油（O/W）乳液中的荧光行为。在本研究中，通过改变添加到油相（O = 1,2-二氯乙烷）和水相中的电解质浓度比来控制O/W乳液滴的平衡伽伐尼电位差（Δφ）。当使用染料的适当组合，即B和C时，我们可以观察到它们的荧光峰强度比从1.08变为1.38，这取决于Δφ从26 mV到73 mV的变化。期望将该方法应用于研究在囊泡或脂质体以及生物膜上发生的电位依赖性离子或电子转移反应。
