Kiran George S, Priyadharsini Sethu, Sajayan Arya, Priyadharsini Gopal B, Poulose Navya, Selvin Joseph
Department of Food Science and Technology, Pondicherry UniversityPuducherry, India.
Department of Microbiology, School of Life Sciences, Pondicherry UniversityPuducherry, India.
Front Microbiol. 2017 Jun 28;8:1138. doi: 10.3389/fmicb.2017.01138. eCollection 2017.
Biosurfactants are smart biomolecules which have wide spread application in medicines, processed foods, cosmetics as well as in bioremediation. In food industry, biosurfactants are used as emulsion stabilizing agents, antiadhesives, and antimicrobial/antibiofilm agents. Nowadays biosurfactant demands in industries has increased tremendously and therefore new bacterial strains are being explored for large scale production of biosurfactants. In this study, an actinobacterial strain MSA31 was isolated from a marine sponge which showed high activity in biosurfactant screening assays such as drop collapsing, oil displacement, lipase and emulsification. Lipopeptide produced by MSA31 was found to be thermostable which was evident in differential scanning calorimetry analysis. The spectral data obtained in the Fourier transform infrared spectroscopy showed the presence of aliphatic groups combined with peptide moiety which is a characteristic feature of lipopeptides. The stability index of lipopeptide MSA31 revealed "halo-alkali and thermal tolerant biosurfactant" which can be used in the food industry. Microtiter plate assay showed 125 μg/ml of lipopeptide was effective in reducing the biofilm formation activity of pathogenic multidrug resistant . The confocal laser scanning microscopic images provided further evidences that lipopeptide MSA31 was an effective antibiofilm agent. The antioxidant activity of lipopeptide MSA31 may be due to the presence of unsaturated fatty acid present in the molecule. The brine shrimp cytotoxicity assay showed lipopeptide MSA31 was non-toxic and can be used as food additives. Incorporation of lipopeptide MSA31 in muffin showed improved organoleptic qualities compared to positive and negative control. This study provides a valuable input for this lipopeptide to be used in food industry as an effective emulsifier, with good antioxidant activity and as a protective agent against .
生物表面活性剂是一类智能生物分子，在医药、加工食品、化妆品以及生物修复等领域有着广泛的应用。在食品工业中，生物表面活性剂可用作乳化稳定剂、抗黏附剂以及抗菌/抗生物膜剂。如今，工业对生物表面活性剂的需求大幅增加，因此人们正在探索新的细菌菌株以大规模生产生物表面活性剂。在本研究中，从一种海洋海绵中分离出一株放线菌菌株MSA31，该菌株在诸如液滴塌陷、油置换、脂肪酶和乳化等生物表面活性剂筛选试验中表现出高活性。发现MSA31产生的脂肽具有热稳定性，这在差示扫描量热分析中很明显。傅里叶变换红外光谱获得的光谱数据显示存在与肽部分结合的脂肪族基团，这是脂肽的一个特征。脂肽MSA31的稳定性指数显示其为“耐盐碱性和耐热性生物表面活性剂”，可用于食品工业。微量滴定板试验表明，125μg/ml的脂肽可有效降低致病性多重耐药菌的生物膜形成活性。共聚焦激光扫描显微镜图像进一步证明脂肽MSA31是一种有效的抗生物膜剂。脂肽MSA31的抗氧化活性可能归因于分子中存在的不饱和脂肪酸。卤虫细胞毒性试验表明脂肽MSA31无毒，可作为食品添加剂。与阳性和阴性对照相比，在松饼中加入脂肽MSA31显示出感官品质得到改善。本研究为该脂肽作为一种有效的乳化剂、具有良好的抗氧化活性并作为针对……的保护剂应用于食品工业提供了有价值的信息。
