Combination of Metabolomic and Proteomic Analysis Revealed Different Features among Subspecies and Strains While Testing in the Model Organism Highlighted Probiotic Properties.

represents a technologically relevant member of lactic acid bacteria, since the two subspecies and are widely associated with fermented dairy products. In the present work, we report the characterization of two commercial strains belonging to subspecies , and a novel strain previously isolated from a traditional fermented fresh cheese. A phenomic approach was performed by combining metabolomic and proteomic analysis of the three strains, which were subsequently supplemented as food source to the model organism , with the final aim to evaluate their possible probiotic effects. Restriction analysis of 16S ribosomal DNA revealed that the novel foodborne strain belonged to subspecies . Proteomic and metabolomic approaches showed differences in folate, aminoacid and sugar metabolic pathways among the three strains. Moreover, evaluation of lifespan, larval development, brood size, and bacterial colonization capacity demonstrated that subsp. diet exerted beneficial effects on nematodes. On the other hand, both subsp. strains affected lifespan and larval development. We have characterized three strains belonging to subspecies and highlighting their divergent origin. In particular, the two closely related isolates subspecies display different galactose metabolic capabilities. Moreover, the subspecies strain demonstrated potential probiotic features. Combination of platforms coupled with screening in the simple model organism is a powerful tool to characterize industrially relevant bacterial isolates.