School of Biological Sciences, University of Essex, Wivenhoe Park, Colchester, CO4 3SQ, UK.
Metallomics. 2017 Nov 15;9(11):1470-1482. doi: 10.1039/c7mt00146k.
Copper nitrite reductases (CuNiR) carry out the first committed step of the denitrification pathway of the global nitrogen cycle, the reduction of nitrite (NO) to nitric oxide (NO). As such, they are of major agronomic and environmental importance. CuNiRs occur primarily in denitrifying soil bacteria which carry out the overall reduction of nitrate to dinitrogen. In this article, we review the insights gained into copper nitrite reductase (CuNiR) function from three dimensional structures. We particularly focus on developments over the last decade, including insights from serial femtosecond crystallography using X-ray free electron lasers (XFELs) and from the recently discovered 3-domain CuNiRs.
亚硝酸盐还原酶(CuNiR)进行全球氮循环反硝化途径的第一步,将亚硝酸盐(NO)还原为一氧化氮(NO)。因此,它们具有重要的农业和环境意义。CuNiRs 主要存在于进行硝酸盐整体还原为氮气的反硝化土壤细菌中。在本文中,我们从三维结构综述了对铜亚硝酸盐还原酶(CuNiR)功能的深入了解。我们特别关注过去十年的发展,包括使用 X 射线自由电子激光(XFEL)的连续飞秒晶体学和最近发现的 3 结构域 CuNiRs 的见解。