Martini F, Bossa F, Barra D
Peptides. 1985;6 Suppl 3:103-5. doi: 10.1016/0196-9781(85)90358-4.
Assay of pyroglutamyl aminopeptidase by HPLC methodology allows determination of the kinetic parameters of the enzyme for a variety of natural peptide substrates. Moreover, by this method it is possible both to evaluate the extent of the enzymatic reaction by determining the amount of pyrrolidone carboxylic acid released and to obtain the unblocked peptide for structural and functional characterization.
采用高效液相色谱法测定焦谷氨酸氨肽酶，可确定该酶作用于多种天然肽底物时的动力学参数。此外，通过这种方法，既能通过测定释放的吡咯烷酮羧酸的量来评估酶促反应的程度，又能获得用于结构和功能表征的未封闭肽。
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