采用高效液相色谱-电化学检测法分析低水平γ-谷氨酰转移酶活性。
The analysis of low levels of gamma-glutamyltransferase activity by high-performance liquid chromatography with electrochemical detection.
作者信息
Hoskins J A, Davies F F
出版信息
Anal Biochem. 1986 Feb 1;152(2):314-8. doi: 10.1016/0003-2697(86)90415-x.
A method for the analysis of low levels of the enzyme gamma-glutamyltransferase in biological samples by high-performance liquid chromatography with electrochemical detection is described. A gamma-glutamyl moiety from glutathione is transferred by the enzyme to glycylglycine to produce a tripeptide which is assayed directly after a purification step using octadecylsilica. Confirmation of the method is by use of the inhibitor AT-125. The method is used to measure the level of enzyme activity in rodent tissues and in cultured cells.
本文描述了一种通过高效液相色谱-电化学检测法分析生物样品中低水平γ-谷氨酰转移酶的方法。谷胱甘肽中的γ-谷氨酰部分被该酶转移至甘氨酰甘氨酸，生成一种三肽，经使用十八烷基硅胶的纯化步骤后直接进行测定。该方法通过使用抑制剂AT-125进行验证。此方法用于测定啮齿动物组织和培养细胞中的酶活性水平。
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