Martinez-Royo Albert, Alabart José Luis, Sarto Pilar, Serrano Magdalena, Lahoz Belén, Folch José, Calvo Jorge Hugo
Unidad de Producción y Sanidad Animal, Centro de Investigación y Tecnología Agroalimentaria de Aragón (CITA), Gobierno de Aragón, Av. Montañana 930, 50059 Zaragoza, Spain.
Unidad de Producción y Sanidad Animal, Centro de Investigación y Tecnología Agroalimentaria de Aragón (CITA), Gobierno de Aragón, Av. Montañana 930, 50059 Zaragoza, Spain; Instituto Agroalimentario de Aragón - IA2 (CITA-Universidad de Zaragoza), Spain.
Theriogenology. 2017 Sep 1;99:21-29. doi: 10.1016/j.theriogenology.2017.05.011. Epub 2017 May 19.
Sheep breeds from Mediterranean area show reproductive seasonal patterns of oestrous behaviour and ovulatory activity, mainly regulated by variation in the photoperiod. Maximal reproductive activity is associated with short days from August to March. The aim of this study therefore was, to identify new SNPs and genes associated to reproductive seasonality in sheep by using the Illumina OvineSNP50 Beadchip. A total of 239 adult Rasa Aragonesa breed ewes from one flock were controlled from January to August. Three reproductive seasonality traits were considered: the total days of anoestrus (TDA), based on weekly individual plasma progesterone levels and defined as the sum of days in anoestrus, considering anoestrus those periods with three or more consecutive P4 concentrations lower than 0.5 ng/ml; the progesterone cycling months (P4CM), defined for each ewe as the rate of cycling months between January and August based on progesterone determinations and the oestrus cycling months (OCM), defined for each ewe as the rate of months cycling between January and August based on oestrus records. Genotyping of 123 ewes was performed with the OvineSNP50 Infinium Beadchip. After the quality control (QC) performed on the raw genotypes, a total of 47,206 SNPs distributed over the 27 ovine chromosomes and 110 ewes were included in subsequent analyses. Principal component analysis revealed a substructure within the total dataset and identified 4 principal clusters in the experimental flock. None of the SNPs overcame the genome-wide significance level (P = 1.06 × 10). However, the SNPs OAR4_66002395 (9.41E-6), and OAR8_25877010 (1.86E-5) reached the genome-wide suggestive significance level (set to 2.32 × 10) for TDA and P4CM traits, respectively, while OAR23_14608581 was significant for both TDA (2.02E-5) and P4CM (1.05E-5) traits. Five SNPs evidenced association at chromosome-wise level: SNPs OAR4_66002395, OAR23_14608581 and s20800 (DTA), and OAR8_25877010, OAR23_14608581 and s48474 (P4CM). Several genes related to circadian and circannual rhythms were found close to these SNPs: NPSR1 (SNP OAR4_66002395), HS3ST5 (SNP OAR8_25877010), RPTOR (SNP s48474), and NPTX1 (SNP s48474) and could be considered as candidate gene related to TDA and P4CM traits.
来自地中海地区的绵羊品种表现出发情行为和排卵活动的繁殖季节性模式，主要受光周期变化的调节。最大繁殖活动与8月至次年3月的短日照有关。因此，本研究的目的是通过使用Illumina OvineSNP50 Beadchip芯片来鉴定与绵羊繁殖季节性相关的新单核苷酸多态性（SNP）和基因。从1月到8月对来自一个羊群的239只成年阿拉贡拉萨品种母羊进行了监测。考虑了三个繁殖季节性性状：基于每周个体血浆孕酮水平的总乏情天数（TDA），定义为乏情期天数之和，将连续三个或更多P4浓度低于0.5 ng/ml的时期视为乏情期；孕酮循环月数（P4CM），基于孕酮测定为每只母羊定义为1月至8月间的循环月数比例；发情循环月数（OCM），基于发情记录为每只母羊定义为1月至8月间的循环月数比例。使用OvineSNP50 Infinium Beadchip芯片对123只母羊进行基因分型。在对原始基因型进行质量控制（QC）后，分布在27条绵羊染色体上的总共47,206个SNP和110只母羊被纳入后续分析。主成分分析揭示了整个数据集中的一个亚结构，并在实验羊群中识别出4个主要聚类。没有一个SNP超过全基因组显著性水平（P = 1.06 × 10）。然而，SNP OAR4_66002395（9.41E - 6）和OAR8_25877010（1.86E - 5）分别针对TDA和P4CM性状达到了全基因组暗示性显著性水平（设定为2.3 × 10），而OAR23_14608581对TDA（2.02E - 5）和P4CM（1.05E - 5）性状均显著。五个SNP在染色体水平上显示出关联：SNP OAR4_66002395、OAR23_14608581和s20800（DTA），以及OAR8_25877010、OAR23_14608581和s48474（P4CM）。在这些SNP附近发现了几个与昼夜节律和年节律相关的基因：神经肽S受体1（NPSR1，SNP OAR4_66002395）、硫酸乙酰肝素3 - O - 磺基转移酶5（HS3ST5，SNP OAR8_25877010）、雷帕霉素靶蛋白（RPTOR，SNP s48474）和神经肽毒素1（NPTX1）（SNP s48474），它们可被视为与TDA和P4CM性状相关的候选基因。
