拟南芥中的AtVps11对胚胎中的液泡生物发生至关重要，并参与花粉管生长。
AtVps11 is essential for vacuole biogenesis in embryo and participates in pollen tube growth in Arabidopsis.
作者信息
Tan Xiaoyun, Wei Juan, Li Beibei, Wang Mengru, Bao Yiqun
机构信息
College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, People's Republic of China.
College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, People's Republic of China.
出版信息
Biochem Biophys Res Commun. 2017 Sep 23;491(3):794-799. doi: 10.1016/j.bbrc.2017.07.059. Epub 2017 Jul 12.
Vacuoles are multiple functional and essential organelles in plants. Studies in Saccharomyces cerevisiae had identified a tethering factor HOPS (Homotypic Fusion and Vacuolar Protein Sorting) complex that plays a critical role in vacuole biogenesis. The HOPS complex consists of four core subunits (Vps11, Vps16, Vps18 and Vps33) and two special subunits (Vps39 and Vps41). All these subunits were found in Arabidopsis, and our knowledge of the function of Arabidopsis HOPS complex are still limited. In this study, we investigated the function of AtVps11 gene in Arabidopsis, we found that vps11/- lead to embryo lethal, vacuole biogenesis in embryo was impaired. Furthermore, pollen tube growth was arrested by vps11 mutation, however, no obvious vacuole biogenesis defects were found in vps11 pollen tube. Our study indicated that in Arabidopsis, Vps11 is required for vacuole biogenesis in embryo, which is essential for embryogenesis. It also plays a role in pollen tube growth but looks not required for vacuole biogenesis in pollen tube.
液泡是植物中具有多种功能的重要细胞器。对酿酒酵母的研究已鉴定出一种拴系因子HOPS（同型融合和液泡蛋白分选）复合体，其在液泡生物发生中起关键作用。HOPS复合体由四个核心亚基（Vps11、Vps16、Vps18和Vps33）和两个特殊亚基（Vps39和Vps41）组成。在拟南芥中发现了所有这些亚基，但我们对拟南芥HOPS复合体功能的了解仍然有限。在本研究中，我们研究了拟南芥AtVps11基因的功能，发现vps11/-导致胚胎致死，胚胎中的液泡生物发生受损。此外，vps11突变使花粉管生长停滞，然而，在vps11花粉管中未发现明显的液泡生物发生缺陷。我们的研究表明，在拟南芥中，Vps11是胚胎液泡生物发生所必需的，这对胚胎发育至关重要。它在花粉管生长中也起作用，但似乎不是花粉管液泡生物发生所必需的。
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