Department of Biomedical Sciences, College of Veterinary Medicine, Oregon State University, Corvallis, OR, 97331, USA.
Seafood Research and Education Center, Oregon State University, Astoria, OR, 97103, USA.
Microb Ecol. 2018 Jan;75(1):152-162. doi: 10.1007/s00248-017-1025-8. Epub 2017 Jul 17.
The human pathogen Vibrio parahaemolyticus is a leading cause of seafood-borne illness in the USA, and infections with V. parahaemolyticus typically result from eating raw or undercooked oysters. V. parahaemolyticus has been shown to be highly resistant to oyster depuration, suggesting that the bacterium possesses specific mechanisms or factors for colonizing oysters and persisting during depuration. In this study, we characterized eight different V. parahaemolyticus strains for differences in resistance to oyster depuration, biofilm formation, and motility. While each strain exhibited distinct phenotypes in the various assays, we determined that biofilm formation on abiotic surfaces, such as glass or plastic, does not directly correlate with bacterial retention in oysters during depuration. However, we did observe that the motility phenotype of a strain appeared to be a better indicator for persistence in the oyster. Further studies examining the molecular mechanisms underlying the observed colonization differences by these and other V. parahaemolyticus strains may provide beneficial insights into what critical factors are required for proficient colonization of the Pacific oyster.
人类病原体副溶血性弧菌是美国食源性疾病的主要致病菌，感染副溶血性弧菌通常是由于食用生的或未煮熟的牡蛎。副溶血性弧菌对牡蛎净化具有高度抗性，这表明该细菌具有定植牡蛎和在净化过程中持续存在的特定机制或因素。在这项研究中，我们对八种不同的副溶血性弧菌菌株进行了特征描述，以研究它们对牡蛎净化、生物膜形成和运动性的抗性差异。虽然每个菌株在各种检测中表现出不同的表型，但我们确定在非生物表面（如玻璃或塑料）上形成生物膜与净化过程中细菌在牡蛎中的保留并不直接相关。然而，我们确实观察到，菌株的运动表型似乎是其在牡蛎中持续存在的更好指标。进一步研究这些和其他副溶血性弧菌菌株观察到的定植差异的分子机制可能为深入了解在太平洋牡蛎中定植所需的关键因素提供有益的见解。
