Jiao Ya, Wang Xiao, Zhang Jixun, Qi Yongjun, Gong Hongmin, Jiang Duyin
Department of Emergency and Department of Burns and Plastic Surgery, The Second Hospital of Shandong University, Jinan, Shandong, 250033, People's Republic of China.
School of Medicine, Shandong University, Jinan, Shandong, 250012, People's Republic of China.
Stem Cell Res Ther. 2017 Jul 18;8(1):170. doi: 10.1186/s13287-017-0624-0.
Keloid is one kind of benign skin disease caused by hyperplasia of fibroblasts and collagen fibrils. It is refractory due to the lack of an effective treatment at present, which puts pressure on seeking a new therapeutic regimen. Mesenchymal stem cells (MSCs) from fetal skin are considered to play a crucial role in scarless healing. Nevertheless, the efficacy of them in keloid disorders remains poorly understood.
Keloid fibroblasts (KFs), human adult dermal fibroblasts (ADFs), and human fetal dermal mesenchymal stem cells (FDMSCs) were isolated to single cells and cultured in Dulbecco's modified Eagle's medium (DMEM). ADFs and FDMSCs were used to generate ADF-conditioned medium (A-CM) and FDMSC-conditioned medium (F-CM). The effects of A-CM and F-CM on KFs were tested using MTT assay, BrdU assay, TUNEL assay, quantitative polymerase chain reaction, Western blot, and annexin V-FITC/PI binding assay,.
FDMSCs inhibited the bioactivity of KFs, downregulated the expression of the antiapoptotic protein BCL-2, and upregulated the expression of the proapoptotic protein BAX of KFs by secreting some soluble substances, thus accelerating the apoptosis of KFs.
F-CM induces apoptosis of KFs, providing a novel treatment strategy for keloid disorders.
瘢痕疙瘩是一种由成纤维细胞和胶原纤维增生引起的良性皮肤病。由于目前缺乏有效的治疗方法，该病难以治愈，这给寻求新的治疗方案带来了压力。胎儿皮肤间充质干细胞（MSCs）被认为在无瘢痕愈合中起关键作用。然而，它们在瘢痕疙瘩疾病中的疗效仍知之甚少。
将瘢痕疙瘩成纤维细胞（KFs）、成人真皮成纤维细胞（ADFs）和人胎儿真皮间充质干细胞（FDMSCs）分离为单细胞，并在杜氏改良 Eagle 培养基（DMEM）中培养。使用 ADFs 和 FDMSCs 分别制备 ADF 条件培养基（A-CM）和 FDMSC 条件培养基（F-CM）。采用 MTT 法、BrdU 法、TUNEL 法、定量聚合酶链反应、蛋白质免疫印迹法和膜联蛋白 V-FITC/PI 结合法检测 A-CM 和 F-CM 对 KFs 的影响。
FDMSCs 通过分泌一些可溶性物质抑制 KFs 的生物活性，下调抗凋亡蛋白 BCL-2 的表达，并上调 KFs 促凋亡蛋白 BAX 的表达，从而加速 KFs 的凋亡。
F-CM 诱导 KFs 凋亡，为瘢痕疙瘩疾病提供了一种新的治疗策略。
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