Ledesma Alba, Zalazar Lucía, Fernández-Alegre Estela, Hozbor Federico, Cesari Andreina, Martínez-Pastor Felipe
Universidad Nacional de Mar del Plata, Ruta 226 km 73.5, 7620, Balcarce, Argentina; Biotecnología de la Reproducción, Departamento de Producción Animal, Instituto Nacional de Tecnología Agropecuaria (INTA), Ruta 226 km 73.5, 7620, Balcarce, Argentina.
Biología de Microorganismos y Gametas, Instituto de Investigaciones Biológicas-CONICET, Universidad Nacional de Mar del Plata, Funes 3250, 7600, Mar del Plata, Argentina.
Anim Reprod Sci. 2017 Sep;184:44-50. doi: 10.1016/j.anireprosci.2017.06.015. Epub 2017 Jun 27.
Any physiological mechanism involved in sperm selection and semen improvement has effects on heterogeneous sperm populations. This is mainly due to the fact that sperm populations within a single ejaculate have considerable heterogeneity for many variables, such as motility which is meaningful in terms of understanding how some sperm cells possess fertility advantages as compared with other cells. In the present research, initially there was a multivariate and clustering analysis used to assess sperm motility data from cryopreserved ram semen to identify subpopulations and compare the distribution of these clusters between rams with lesser and greater fertility. There were four classifications made of sperm subpopulations (clusters): CL1 fast/linear/progressive sperm; CL2 fast/non-linear sperm; CL3 very fast/linear sperm with vigorous beating and CL4 slow/non-linear sperm. Rams with greater fertility had a lesser proportion of sperm considered as "hyperactivated" (CL2) and a greater proportion of slow and non-linear sperm (CL4) than sperm of rams with lesser fertility. In addition, the effects were assessed for the capacity of seminal plasma (SP) and interacting SP proteins (iSPP) that were present during different seasons of the year to improve the distribution of sperm within subpopulations of semen from rams with lesser fertility. The iSPP and SP were obtained by artificial vagina (AV) and electroejaculation (EE) during breeding and non-breeding seasons and added to thawed semen. All the aggregates had a significant effect on the distribution of sperm subpopulations and effects differed among seasons of the year and depending on collection method used. Even though, future studies are needed to assess the contribution of each subpopulation on ram sperm fertility, it is important that a multivariate analysis be used to evaluate the effect of a treatment on sperm quality variables.
任何参与精子筛选和精液改善的生理机制都会对异质性精子群体产生影响。这主要是因为在单次射精中的精子群体在许多变量上具有相当大的异质性，例如活力，这对于理解某些精子细胞与其他细胞相比如何具有生育优势具有重要意义。在本研究中，最初使用多变量和聚类分析来评估冷冻保存的公羊精液的精子活力数据，以识别亚群并比较生育力较低和较高的公羊之间这些聚类的分布。精子亚群（聚类）分为四类：CL1快速/线性/渐进性精子；CL2快速/非线性精子；CL3具有强烈跳动的非常快速/线性精子和CL4缓慢/非线性精子。生育力较高的公羊中被认为“超活化”（CL2）的精子比例较低，而缓慢和非线性精子（CL4）的比例高于生育力较低的公羊的精子。此外，还评估了一年中不同季节存在的精浆（SP）和相互作用的SP蛋白（iSPP）改善生育力较低的公羊精液亚群内精子分布的能力。在繁殖季节和非繁殖季节通过人工阴道（AV）和电射精（EE）获得iSPP和SP，并添加到解冻的精液中。所有这些聚集体对精子亚群的分布都有显著影响，并且影响因一年中的季节和所使用的采集方法而异。尽管未来需要进行研究来评估每个亚群对公羊精子生育力的贡献，但使用多变量分析来评估治疗对精子质量变量的影响很重要。
