一种来自海胆卵的微管激活ATP酶，与细胞质动力蛋白和驱动蛋白不同。
A microtubule-activated ATPase from sea urchin eggs, distinct from cytoplasmic dynein and kinesin.
作者信息
Collins C A, Vallee R B
出版信息
Proc Natl Acad Sci U S A. 1986 Jul;83(13):4799-803. doi: 10.1073/pnas.83.13.4799.
Abstract
We report an ATPase activity, present in sea urchin egg cytosol, that is activated by microtubules. The activity sediments at 10 S in sucrose gradients and is clearly distinct from activities at 12 S and 20 S due to cytoplasmic dynein. Potent activation of the ATPase is observed when endogenous egg tubulin is induced to assemble with taxol or when exogenous taxol-stabilized pure brain tubulin microtubules or flagellar outer-doublet microtubules are added. No activation by tubulin subunits or taxol alone is detectable. In contrast to flagellar or cytoplasmic dynein, the microtubule-activated enzyme is unaffected by vanadate or by nonionic detergents and hydrolyzes GTP in addition to ATP. In contrast to kinesin, it cosediments with microtubules in the presence or absence of ATP. The microtubule-activated enzyme may have a role in microtubule-based motility.
摘要
我们报道了一种存在于海胆卵细胞质溶胶中的ATP酶活性，该活性可被微管激活。该活性在蔗糖梯度中沉降系数为10S，与由于细胞质动力蛋白导致的12S和20S处的活性明显不同。当内源性卵微管蛋白被紫杉醇诱导组装时，或者当添加外源性紫杉醇稳定的纯脑微管蛋白微管或鞭毛外双联微管时，可观察到ATP酶的强力激活。单独的微管蛋白亚基或紫杉醇均未检测到激活作用。与鞭毛或细胞质动力蛋白不同，微管激活的酶不受钒酸盐或非离子去污剂的影响，除了ATP外还能水解GTP。与驱动蛋白不同，无论有无ATP，它都与微管共沉降。微管激活的酶可能在基于微管的运动中起作用。
相似文献
1
A microtubule-activated ATPase from sea urchin eggs, distinct from cytoplasmic dynein and kinesin.一种来自海胆卵的微管激活ATP酶，与细胞质动力蛋白和驱动蛋白不同。
Proc Natl Acad Sci U S A. 1986 Jul;83(13):4799-803. doi: 10.1073/pnas.83.13.4799.
2
Characterization of the sea-urchin egg microtubule-activated ATPase.海胆卵微管激活的ATP酶的特性分析
J Cell Sci Suppl. 1986;5:197-204. doi: 10.1242/jcs.1986.supplement_5.13.
3
Dynein isoforms in sea urchin eggs.海胆卵中的动力蛋白异构体。
J Biol Chem. 1988 May 15;263(14):6759-71.
4
Correlation between the ATPase and microtubule translocating activities of sea urchin egg kinesin.海胆卵驱动蛋白的ATP酶活性与微管转运活性之间的相关性。
Nature. 1987;328(6126):160-3. doi: 10.1038/328160a0.
5
Isolation of microtubules and a dynein-like MgATPase from unfertilized sea urchin eggs.从未受精的海胆卵中分离微管和一种类似动力蛋白的镁ATP酶。
J Biol Chem. 1984 May 25;259(10):6516-25.
6
Characterization of the microtubule-activated ATPase of brain cytoplasmic dynein (MAP 1C).脑细胞质动力蛋白（MAP 1C）的微管激活ATP酶的特性分析。
J Cell Biol. 1988 Sep;107(3):1001-9. doi: 10.1083/jcb.107.3.1001.
7
Dynein-like Mg2+-ATPase in mitotic spindles isolated from sea urchin embryos (Strongylocentrotus droebachiensis).从海胆胚胎（强壮球海胆）中分离出的有丝分裂纺锤体中的类动力蛋白Mg2 + -ATP酶。
J Cell Biol. 1980 Sep;86(3):738-45. doi: 10.1083/jcb.86.3.738.
8
Comparison of the motile and enzymatic properties of two microtubule minus-end-directed motors, ncd and cytoplasmic dynein.两种微管负端定向马达蛋白ncd和细胞质动力蛋白的运动及酶活性特性比较。
Biochemistry. 1995 Feb 7;34(5):1575-82. doi: 10.1021/bi00005a013.
9
Quantitation of the dynein pool in unfertilized sea urchin eggs.未受精海胆卵中动力蛋白总量的定量分析。
Biochim Biophys Acta. 1989 Jan 27;990(1):31-9. doi: 10.1016/s0304-4165(89)80008-x.
10
Two distinct isoforms of sea urchin egg dynein.海胆卵动力蛋白的两种不同同工型。
Cell Motil Cytoskeleton. 1992;21(4):281-92. doi: 10.1002/cm.970210404.
引用本文的文献
1
Live-cell imaging in Caenorhabditis elegans reveals the distinct roles of dynamin self-assembly and guanosine triphosphate hydrolysis in the removal of apoptotic cells.秀丽隐杆线虫活细胞成像揭示了在凋亡细胞清除过程中，GTP 水解和动力蛋白自组装的不同作用。
Mol Biol Cell. 2010 Feb 15;21(4):610-29. doi: 10.1091/mbc.e09-05-0440. Epub 2009 Dec 16.
2
Kinesin-5 in Drosophila embryo mitosis: sliding filament or spindle matrix mechanism?果蝇胚胎有丝分裂中的驱动蛋白-5：滑动丝机制还是纺锤体基质机制？
Cell Motil Cytoskeleton. 2009 Aug;66(8):500-8. doi: 10.1002/cm.20349.
3
Analysis of cytoskeletal and motility proteins in the sea urchin genome assembly.海胆基因组组装中细胞骨架和运动蛋白的分析。
Dev Biol. 2006 Dec 1;300(1):219-37. doi: 10.1016/j.ydbio.2006.08.052. Epub 2006 Aug 26.
4
Characterization of the microtubule-activated ATPase of brain cytoplasmic dynein (MAP 1C).脑细胞质动力蛋白（MAP 1C）的微管激活ATP酶的特性分析。
J Cell Biol. 1988 Sep;107(3):1001-9. doi: 10.1083/jcb.107.3.1001.
5
MAP 1C is a microtubule-activated ATPase which translocates microtubules in vitro and has dynein-like properties.微管相关蛋白1C是一种微管激活的ATP酶，它在体外能使微管移位，并具有类似动力蛋白的特性。
J Cell Biol. 1987 Sep;105(3):1273-82. doi: 10.1083/jcb.105.3.1273.
6
Identification of a novel nucleotide-sensitive microtubule-binding protein in HeLa cells.在HeLa细胞中鉴定一种新型核苷酸敏感微管结合蛋白。
J Cell Biol. 1990 May;110(5):1623-33. doi: 10.1083/jcb.110.5.1623.
本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
Reassembly of flagellar B (alpha beta) tubulin into singlet microtubules: consequences for cytoplasmic microtubule structure and assembly.鞭毛B（αβ）微管蛋白重新组装成单微管：对细胞质微管结构和组装的影响。
J Cell Biol. 1981 May;89(2):323-37. doi: 10.1083/jcb.89.2.323.
3
Nucleotide requirements for anaphase chromosome movements in permeabilized mitotic cells: anaphase B but not anaphase A requires ATP.通透有丝分裂细胞后期染色体运动的核苷酸需求：后期B需要ATP，而后期A不需要。
Cell. 1982 Jan;28(1):15-22. doi: 10.1016/0092-8674(82)90370-1.
4
Dynein-like Mg2+-ATPase in mitotic spindles isolated from sea urchin embryos (Strongylocentrotus droebachiensis).从海胆胚胎（强壮球海胆）中分离出的有丝分裂纺锤体中的类动力蛋白Mg2 + -ATP酶。
J Cell Biol. 1980 Sep;86(3):738-45. doi: 10.1083/jcb.86.3.738.
5
The identification of a dynein ATPase in unfertilized sea urchin eggs.未受精海胆卵中动力蛋白ATP酶的鉴定。
Dev Biol. 1980 Feb;74(2):364-78. doi: 10.1016/0012-1606(80)90438-8.
6
Adenosine triphosphatase activity of bovine brain microtubule protein.牛脑微管蛋白的三磷酸腺苷酶活性
J Biol Chem. 1980 Jan 25;255(2):486-91.
7
Identity and origin of the ATPase activity associated with neuronal microtubules. II. Identification of a 50,000-dalton polypeptide with ATPase activity similar to F-1 ATPase from mitochondria.与神经元微管相关的ATP酶活性的特性及起源。II. 一种50,000道尔顿具有ATP酶活性的多肽的鉴定，其类似于线粒体中的F-1 ATP酶。
J Cell Biol. 1983 May;96(5):1306-15. doi: 10.1083/jcb.96.5.1306.
8
Identity and Origin of the ATPase activity associated with neuronal microtubules. I. The ATPase activity is associated with membrane vesicles.与神经元微管相关的ATP酶活性的特性与起源。I. ATP酶活性与膜泡相关。
J Cell Biol. 1983 May;96(5):1298-305. doi: 10.1083/jcb.96.5.1298.
9
Cytoplasmic dynein of the sea urchin egg. II. Purification, characterization and interactions with microtubules and Ca-calmodulin.海胆卵的细胞质动力蛋白。II. 纯化、特性鉴定及其与微管和钙调蛋白的相互作用
J Biochem. 1983 Jan;93(1):87-98. doi: 10.1093/oxfordjournals.jbchem.a134182.
10
Presence of two distinct adenosine triphosphatase activities in bovine brain microtubules.牛脑微管中两种不同的三磷酸腺苷酶活性的存在。
FEBS Lett. 1982 Jul 19;144(1):112-6. doi: 10.1016/0014-5793(82)80581-4.
Zotero 插件