Chin Clament Fui Seung, Furuya Yoshihide, Zainudin Mohd Huzairi Mohd, Ramli Norhayati, Hassan Mohd Ali, Tashiro Yukihiro, Sakai Kenji
Laboratory of Soil and Environmental Microbiology, Graduate School of Bioresource and Bioenvironmental Sciences, Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan; Laboratory of Microbiology, Faculty of Sustainable Agriculture, Universiti Malaysia Sabah, Sandakan Campus, Locked Bag No. 3, 90509 Sandakan, Sabah, Malaysia.
Laboratory of Soil and Environmental Microbiology, Graduate School of Bioresource and Bioenvironmental Sciences, Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.
J Biosci Bioeng. 2017 Nov;124(5):506-513. doi: 10.1016/j.jbiosc.2017.05.016. Epub 2017 Jul 20.
Previously, a unique co-compost produced by composting empty fruit bunch with anaerobic sludge from palm oil mill effluent, which contributed to establishing a zero-emission industry in Malaysia. Little was known about the bacterial functions during the composting process and fertilization capacity of this co-compost. We isolated 100 strains from the co-compost on 7 types of enumeration media and screened 25 strains using in vitro tests for 12 traits, grouping them according to three functions: plant growth promoting (fixation of nitrogen; solubilization of phosphorus, potassium, and silicate; production of 3-indoleacetic acid, ammonia, and siderophore), biocontrolling (production of chitinase and anti-Ganoderma activity), and composting (degradation of lignin, xylan, and cellulose). Using 16S rRNA gene sequence analysis, 25 strains with strong or multi-functional traits were found belong to the genera Bacillus, Paenibacillus, Citrobacter, Enterobacter, and Kosakonia. Furthermore, several strains of Citrobacter sedlakii exhibited a plant growth-stimulation in vivo komatsuna plant cultivation test. In addition, we isolated several multifunctional strains; Bacillus tequilensis CE4 (biocontrolling and composting), Enterobacter cloacae subsp. dissolvens B3 (plant growth promoting and biocontrolling), and C. sedlakii CESi7 (plant growth promoting and composting). Some bacteria in the co-compost play significant roles during the composting process and plant cultivation after fertilization, and some multifunctional strains have potential for use in accelerating the biodegradation of lignocellulosic biomass, protecting against Ganoderma boninense infection, and increasing the yield of palm oil.
此前，通过将空果串与棕榈油厂废水的厌氧污泥进行堆肥处理，产生了一种独特的混合堆肥，这有助于在马来西亚建立零排放产业。关于这种混合堆肥在堆肥过程中的细菌功能及其施肥能力，人们了解甚少。我们在7种计数培养基上从混合堆肥中分离出100株菌株，并使用体外试验对25株菌株的12种特性进行了筛选，根据三种功能将它们分组：促进植物生长（固氮；磷、钾和硅酸盐的溶解；3-吲哚乙酸、氨和铁载体的产生）、生物防治（几丁质酶的产生和抗灵芝活性）以及堆肥（木质素、木聚糖和纤维素的降解）。通过16S rRNA基因序列分析，发现25株具有强功能或多功能特性的菌株属于芽孢杆菌属、类芽孢杆菌属、柠檬酸杆菌属、肠杆菌属和科萨科尼亚属。此外，在小松菜植株栽培体内试验中，几种塞德莱基柠檬酸杆菌菌株表现出对植物生长有刺激作用。此外，我们还分离出了几种多功能菌株；特基拉芽孢杆菌CE4（生物防治和堆肥）、阴沟肠杆菌溶亚种B3（促进植物生长和生物防治）以及塞德莱基柠檬酸杆菌CESi7（促进植物生长和堆肥）。混合堆肥中的一些细菌在堆肥过程和施肥后的植物栽培中发挥着重要作用，一些多功能菌株具有加速木质纤维素生物质生物降解、预防灵芝感染以及提高棕榈油产量的潜力。
