Iversen O H, Krajci P, Thoresen G
Virchows Arch B Cell Pathol Incl Mol Pathol. 1986;51(2):115-26. doi: 10.1007/BF02899022.
This study reports the fate of hairless mouse epidermal basal cells arrested in mitosis by a traditional stathmokinetic dose of 0.15 mg Colcemid. Epidermal basal cells in the S phase were labeled with 30 microCi (3H)TdR i.p. After 1 h, four animals from a cage of eight mice were given 0.15 mg Colcemid (Fluka) in 0.5 ml saline, and the other four mice were given saline only. Groups of eight mice (four experimental, four controls) were sacrificed 4, 9, 13, 21 and 25 h after (3H)TdR injection (i.e. 3, 8, 12, 16, 20 and 24 h after Colcemid). The following cell kinetic parameters were determined: the number of labeled basal and suprabasal cells, the mean grain count of the labeled cells, the specific activity, the mitotic count, the number of labeled mitoses, the fraction of labeled mitoses curve and the fraction of cells in S and in G2 as determined by flow cytometry. "Labeled paired twins", i.e. adjoining labeled cells with approximately the same grain count, were also scored. All the results taken together support the conclusion that cells labeled with (3H)TdR and arrested 1 h later with 0.15 mg Colcemid go through at least one subsequent cell division and thereafter some of them move out into the suprabasal layer at a normal rate. Hence, after this dose of Colcemid, cells arrested in mitosis for some hours do not die, and the Colcemid treatment does not seem to produce hyperploid cells. The study confirms the usefulness of this dose of Colcemid as a convenient tool for cell kinetic studies.
本研究报告了用0.15mg秋水仙酰胺的传统有丝分裂阻断剂量使无毛小鼠表皮基底细胞停滞于有丝分裂期后的细胞命运。用30微居里（3H）胸腺嘧啶核苷腹腔注射标记处于S期的表皮基底细胞。1小时后，从一组8只小鼠的笼子中取出4只小鼠，给其腹腔注射0.15mg秋水仙酰胺（Fluka），溶于0.5ml生理盐水中，另外4只小鼠只注射生理盐水。在注射（3H）胸腺嘧啶核苷后4、9、13、21和25小时（即注射秋水仙酰胺后3、8、12、16、20和24小时）处死每组8只小鼠（4只实验组，4只对照组）。测定了以下细胞动力学参数：标记的基底细胞和基底上层细胞数量、标记细胞的平均颗粒计数、比活性、有丝分裂计数、标记有丝分裂数、标记有丝分裂分数曲线以及通过流式细胞术测定的处于S期和G2期的细胞比例。还对“标记的孪生细胞”，即相邻且颗粒计数大致相同的标记细胞进行了计数。综合所有结果支持以下结论：用（3H）胸腺嘧啶核苷标记并在1小时后用0.15mg秋水仙酰胺使其停滞的细胞至少经历一次后续细胞分裂，此后其中一些细胞以正常速率迁移至基底上层。因此，在给予该剂量秋水仙酰胺后，停滞于有丝分裂期数小时的细胞不会死亡，且秋水仙酰胺处理似乎不会产生超倍体细胞。该研究证实了这一剂量的秋水仙酰胺作为细胞动力学研究便捷工具的实用性。
