School of Mechanical and Aerospace Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798, Singapore.
Biomater Sci. 2017 Sep 26;5(10):2056-2067. doi: 10.1039/c7bm00134g.
To decipher specific cell responses to diverse and complex in vivo signals, it is essential to emulate specific surface chemicals, extra cellular matrix (ECM) components and topographical signals through reliable and easily reproducible in vitro systems. However, the effect of multiple cues such as micro-hole/pillar architectures under a common and easily tunable platform remains unexplored. Recently we have demonstrated the positive influence of surface chemical modification of polydimethylsiloxane (PDMS) surfaces on directing long-term adhesion, viability and potency of hMSCs. In this study, we include biophysical signals from diverse surface topographical elements along with biochemical influences to develop a holistic understanding of hMSC responses in complex tissue-like niches. We report the influence of chemically modified PDMS structures encompassing hole-, pillar- and groove-based multi-scale architectures on hMSC morphology, adhesion, proliferation and differentiation. The inclusion of hole and pillar features resulted in enhanced adhesion and proliferation of hMSCs. These effects were more pronounced with the inclusion of grooves, which resulted in the highest osteogenic differentiation among other substrates. Our study provides an additional basis for the chemical/physical regulation of hMSC behavior within controlled biomimetic architectures with an aim to foster efficient tissue regeneration strategies.
为了解析细胞对多样化和复杂的体内信号的特定反应,通过可靠且易于重现的体外系统来模拟特定的表面化学物质、细胞外基质(ECM)成分和拓扑信号至关重要。然而,在共同且易于调节的平台下,多种线索(如微孔/微柱结构)的影响仍有待探索。最近,我们已经证明了对聚二甲基硅氧烷(PDMS)表面进行化学修饰对指导 hMSC 的长期黏附、活力和效力的积极影响。在这项研究中,我们结合了来自不同表面形貌元素的生物物理信号以及生化影响,以全面了解 hMSC 在复杂组织样龛中的反应。我们报告了包含孔、柱和槽等多尺度结构的化学修饰 PDMS 结构对 hMSC 形态、黏附、增殖和分化的影响。孔和柱特征的加入增强了 hMSC 的黏附和增殖。包含槽的效果更为明显,在其他基质中促成了最高的成骨分化。我们的研究为在受控制的仿生结构内通过化学/物理调节 hMSC 行为提供了额外的基础,旨在促进有效的组织再生策略。