Programa de Pós-graduação em Ciências da Saúde-Cardiologia, Instituto de Cardiologia/Fundação Universitária de Cardiologia, Avenida Princesa Isabel, n° 370, 3° andar, Porto Alegre, RS, CEP: 90620-001, Brazil.
Universidade Federal de São Paulo, São José dos Campos, SP, Brazil.
J Transl Med. 2017 Jul 25;15(1):161. doi: 10.1186/s12967-017-1262-0.
In an attempt to increase the therapeutic potential for myocardial regeneration, there is a quest for new cell sources and types for cell therapy protocols. The pathophysiology of heart diseases may affect cellular characteristics and therapeutic results.
To study the proliferative and differentiation potential of mesenchymal stem cells (MSC), isolated from bone marrow (BM) of sternum, we made a comparative analysis between samples of patients with ischemic (IHD) or non-ischemic valvular (VHD) heart diseases. We included patients with IHD (n = 42) or VHD (n = 20), with average age of 60 years and no differences in cardiovascular risk factors. BM samples were collected (16.4 ± 6 mL) and submitted to centrifugation with Ficoll-Paque, yielding 4.5 ± 1.5 × 10 cells/mL.
Morphology, immunophenotype and differentiation ability had proven that the cultivated sternal BM cells had MSC features. The colony forming unit-fibroblast (CFU-F) frequency was similar between groups (p = 0.510), but VHD samples showed positive correlation to plated cells vs. CFU-F number (r = 0.499, p = 0.049). The MSC culture was established in 29% of collected samples, achieved passage 9, without significant difference in expansion kinetics between groups (p > 0.05). Dyslipidemia and the use of statins was associated with culture establishment for IHD patients (p = 0.049 and p = 0.006, respectively).
Together, these results show that the sternum bone can be used as a source for MSC isolation, and that ischemic or valvular diseases do not influence the cellular yield, culture establishment or in vitro growth kinetics.
为了提高心肌再生的治疗潜力，人们一直在寻找新的细胞来源和类型用于细胞治疗方案。心脏病的病理生理学可能会影响细胞特性和治疗结果。
为了研究骨髓（BM）来源的间充质干细胞（MSC）的增殖和分化潜能，我们对缺血性心脏病（IHD）或非缺血性瓣膜性心脏病（VHD）患者的样本进行了比较分析。我们纳入了 IHD（n=42）或 VHD（n=20）患者，平均年龄 60 岁，心血管危险因素无差异。采集 BM 样本（16.4±6 mL）并进行 Ficoll-Paque 离心，得到 4.5±1.5×106 个细胞/mL。
形态学、免疫表型和分化能力证明培养的胸骨 BM 细胞具有 MSC 特征。CFU-F 频率在两组间无差异（p=0.510），但 VHD 组的 plated cells 与 CFU-F 数量呈正相关（r=0.499，p=0.049）。29%的采集样本建立了 MSC 培养，获得 9 代，两组间扩增动力学无显著差异（p>0.05）。IHD 患者的血脂异常和他汀类药物的使用与培养建立相关（p=0.049 和 p=0.006）。
综上所述，这些结果表明胸骨可作为 MSC 分离的来源，缺血性或瓣膜性疾病不影响细胞产量、培养建立或体外生长动力学。
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