酪氨酸羟化酶信使核糖核酸与大鼠肾上腺髓质互补脱氧核糖核酸之间的序列同源性。

Sequence homology between tyrosine hydroxylase mRNA and a rat adrenal medullary cDNA.

作者信息

O'Malley K L

出版信息

J Neurosci Res. 1986;16(1):3-12. doi: 10.1002/jnr.490160103.

Abstract

A rat adrenal medullary recombinant clone has been isolated by cross-hybridization with a tyrosine hydroxylase (TH) cDNA. The new clone has a mRNA size of 5.6 kb and also hybridizes to the 1.9-kb tyrosine hydroxylase message. Southern blot analysis reveals several hybridizing bands in common between the TH cDNA and the adrenal medullary clone. These results demonstrate that the adrenal clone shares sequences in common with the TH gene and/or is closely linked to it in the genome. Hybrid-selected mRNA translation products of the adrenal clone can be immunoprecipitated with dopamine beta hydroxylase antisera. This suggests that the adrenal medullary cDNA may code for another catecholamine pathway enzyme.

摘要

通过与酪氨酸羟化酶（TH）cDNA进行交叉杂交，分离出了一个大鼠肾上腺髓质重组克隆。新克隆的mRNA大小为5.6 kb，并且也与1.9 kb的酪氨酸羟化酶信使RNA杂交。Southern印迹分析显示，TH cDNA和肾上腺髓质克隆之间有几条共同的杂交带。这些结果表明，肾上腺克隆与TH基因共享共同序列，和/或在基因组中与其紧密相连。肾上腺克隆的杂交选择mRNA翻译产物可用多巴胺β羟化酶抗血清进行免疫沉淀。这表明肾上腺髓质cDNA可能编码另一种儿茶酚胺途径酶。