Energy Research Centre of the Netherlands (ECN), Biomass & Energy Efficiency, P.O. Box 1, 1755 ZG Petten, The Netherlands.
Energy Research Centre of the Netherlands (ECN), Biomass & Energy Efficiency, P.O. Box 1, 1755 ZG Petten, The Netherlands.
Bioresour Technol. 2017 Nov;243:994-999. doi: 10.1016/j.biortech.2017.07.072. Epub 2017 Jul 15.
Enzymatic cellulose hydrolysis of pretreated wheat straw pulp to glucose is enhanced when the hydrolysis is performed in the presence of an aqueous extract of the wheat straw. A relative digestibility increase of about 10% has been observed for organosolv, alkaline and dilute acid pretreated wheat straw pulp (enzyme dose 2.5FPU/g pulp). At lower enzyme doses, the extract effect increases leading to an enzyme dose reduction of 40% to obtain a glucose yield of 75% within 48h using organosolv wheat straw pulp. Possibly, cellulase deactivation by irreversible binding to pulp lignin is reduced by competition with proteins in the extract. However, since the extract effect has also been demonstrated for lignin-lean substrates, other effects like improved accessibility of the pulp cellulose (amorphogenesis) cannot be excluded. Overall, this contribution demonstrates the positive effect of biomass extractives on enzymatic cellulose digestibility, thereby reducing costs for 2G biofuels and bio-based chemicals.
当水解在小麦秸秆水提物存在的情况下进行时，预处理的麦草浆的纤维素的酶水解会增强为葡萄糖。对于有机溶剂法、碱性和稀酸预处理的麦草浆（酶剂量 2.5FPU/g 浆），相对消化率提高了约 10%。在较低的酶剂量下，提取物的效果增加，导致使用有机溶剂法麦草浆在 48 小时内获得 75%的葡萄糖产率时，酶剂量减少了 40%。可能是由于提取物中的蛋白质与纤维素酶发生不可逆结合而导致其失活，从而降低了酶的失活。然而，由于提取物的效果也已经在木质素含量低的底物上得到了证明，因此不能排除其他如改善浆纤维素的可及性（无定形化）等效果。总的来说，本研究证明了生物质抽提物对酶促纤维素消化性的积极影响，从而降低了 2G 生物燃料和基于生物的化学品的成本。
