Izdebska Magdalena, Piątkowska-Chmiel Iwona, Korolczuk Agnieszka, Herbet Mariola, Gawrońska-Grzywacz Monika, Gieroba Renata, Sysa Marcin, Czajkowska-Bania Klaudia, Cygal Marcelina, Korga Agnieszka, Dudka Jarosław
a Department of Toxicology, Medical University of Lublin, Chodzki 8, Lublin 20-093, Poland.
b Department of Clinical Pathomorphology, Medical University of Lublin, Jaczewskiego 8b, Lublin 20-090, Poland.
Can J Physiol Pharmacol. 2017 Dec;95(12):1442-1453. doi: 10.1139/cjpp-2016-0561. Epub 2017 Jul 31.
Nonalcoholic fatty liver disease (NAFLD) is currently one of the most common chronic liver diseases, especially in developed countries. One group of substances with a potential use in the treatment of NAFLD are plant polyphenols, represented by resveratrol. The aim of this study was to evaluate the effect of resveratrol on steatosis and oxidative stress in HepG2 cells. The steatosis of cells was carried out using free fatty acids: oleic or palmitic acid and their mixtures. Steatosis was visualized using the intracellular lipid staining by Nile Red dye with a fluorescence microscope. This study also determined the viability of cells and mitochondrial membrane potential. The current study showed that fatty acids and their mixtures induced fat overloading in HepG2 cells. In the group of cells incubated with oleic acid (OA), observed changes were moderate with prevailing micro-vesicular steatosis. In case of cells incubated with palmitic acid (PA) and the mixtures of fatty acids, micro- and macro-vacuolar steatosis occurred in most of the cells. Resveratrol decreased steatosis in HepG2 cells induced by OA, PA, as well as their mixtures, and in most of experimental groups did not reduce cells viability. Resveratrol reduced the oxidative stress in HepG2 cells treated with fatty acids mixtures.
非酒精性脂肪性肝病（NAFLD）是目前最常见的慢性肝病之一，尤其是在发达国家。一类有潜力用于治疗NAFLD的物质是植物多酚，白藜芦醇是其代表。本研究的目的是评估白藜芦醇对HepG2细胞脂肪变性和氧化应激的影响。使用游离脂肪酸：油酸或棕榈酸及其混合物诱导细胞脂肪变性。通过尼罗红染料进行细胞内脂质染色，并用荧光显微镜观察脂肪变性。本研究还测定了细胞活力和线粒体膜电位。当前研究表明，脂肪酸及其混合物会导致HepG2细胞脂肪过载。在用油酸（OA）孵育的细胞组中，观察到的变化较为温和，主要为微泡性脂肪变性。在用棕榈酸（PA）和脂肪酸混合物孵育的细胞中，大多数细胞出现了微泡和大泡性脂肪变性。白藜芦醇可减轻OA、PA及其混合物诱导的HepG2细胞脂肪变性，并且在大多数实验组中不会降低细胞活力。白藜芦醇可减轻用脂肪酸混合物处理的HepG2细胞中的氧化应激。
