从感染杆状病毒载体的昆虫幼虫中纯化重组人酪氨酸酶
Purification of Recombinant Human Tyrosinase from Insect Larvae Infected with the Baculovirus Vector.
作者信息
Dolinska Monika B, Wingfield Paul T, Sergeev Yuri V
机构信息
National Eye Institute, National Institutes of Health, Bethesda, Maryland.
National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, Maryland.
出版信息
Curr Protoc Protein Sci. 2017 Aug 1;89:6.15.1-6.15.12. doi: 10.1002/cpps.37.
Abstract
The purification of an enzyme from insect larvae infected with a baculovirus vector is described. The enzyme tyrosinase is of biomedical importance and catalyzes the first rate-limiting steps in melanin production. Tyrosinase mutations can result in oculocutaneous albinism type 1 (OCA1), an inherited eye disease associated with decreased melanin pigment production and vision defects. To simplify expression and subsequent purification, the extracellular domain is expressed in insect cells, produced in Trichoplusia ni larvae, and purified using affinity and size-exclusion chromatography. The purified recombinant human tyrosinase is a soluble monomeric glycoprotein with an activity that mirrors the tyrosinase in vivo function. © 2017 by John Wiley & Sons, Inc.
摘要
本文描述了从感染杆状病毒载体的昆虫幼虫中纯化一种酶的过程。酪氨酸酶这种酶具有生物医学重要性，它催化黑色素生成过程中的首个限速步骤。酪氨酸酶突变可导致1型眼皮肤白化病（OCA1），这是一种遗传性眼病，与黑色素生成减少和视力缺陷有关。为了简化表达及后续纯化，将细胞外结构域在昆虫细胞中表达，在粉纹夜蛾幼虫中生产，并通过亲和色谱和尺寸排阻色谱进行纯化。纯化后的重组人酪氨酸酶是一种可溶性单体糖蛋白，其活性反映了酪氨酸酶在体内的功能。© 2017约翰威立国际出版公司。
相似文献
1
Purification of Recombinant Human Tyrosinase from Insect Larvae Infected with the Baculovirus Vector.
Curr Protoc Protein Sci. 2017 Aug 1;89:6.15.1-6.15.12. doi: 10.1002/cpps.37.
2
Albinism-causing mutations in recombinant human tyrosinase alter intrinsic enzymatic activity.
PLoS One. 2014 Jan 2;9(1):e84494. doi: 10.1371/journal.pone.0084494. eCollection 2014.
3
Membrane-associated human tyrosinase is an enzymatically active monomeric glycoprotein.
PLoS One. 2018 Jun 5;13(6):e0198247. doi: 10.1371/journal.pone.0198247. eCollection 2018.
4
Oculocutaneous albinism type 1: link between mutations, tyrosinase conformational stability, and enzymatic activity.
Pigment Cell Melanoma Res. 2017 Jan;30(1):41-52. doi: 10.1111/pcmr.12546.
5
The consequences of deglycosylation of recombinant intra-melanosomal domain of human tyrosinase.
Biol Chem. 2017 Dec 20;399(1):73-77. doi: 10.1515/hsz-2017-0178.
6
Characterization of Temperature-Dependent Kinetics of Oculocutaneous Albinism-Causing Mutants of Tyrosinase.
Int J Mol Sci. 2021 Jul 21;22(15):7771. doi: 10.3390/ijms22157771.
7
Production of a recombinant antibody fragment in whole insect larvae.
Mol Biotechnol. 2007 May;36(1):44-51. doi: 10.1007/s12033-007-0014-4.
8
Protein production with recombinant baculoviruses in lepidopteran larvae.
Methods Mol Biol. 2007;388:267-80.
9
In vitro characterization of the intramelanosomal domain of human recombinant TYRP1 and its oculocutaneous albinism type 3-related mutant variants.
Protein Sci. 2023 Jan;32(1):e4518. doi: 10.1002/pro.4518.
10
A novel mutation of the tyrosinase gene causing oculocutaneous albinism type 1 (OCA1).
J Dermatol Sci. 2002 Feb;28(2):102-5. doi: 10.1016/s0923-1811(01)00141-4.
引用本文的文献
1
Synthesis, modelling, and biological evaluation of substituted pyrazole derivatives as potential anti-skin cancer, anti-tyrosinase, and antioxidant agents.
J Enzyme Inhib Med Chem. 2023 Dec;38(1):2205042. doi: 10.1080/14756366.2023.2205042.
2
In Vitro Reconstitution of the Melanin Pathway's Catalytic Activities Using Tyrosinase Nanoparticles.
Int J Mol Sci. 2022 Dec 30;24(1):639. doi: 10.3390/ijms24010639.
3
Protein Biochemistry and Molecular Modeling of the Intra-Melanosomal Domain of Human Recombinant Tyrp2 Protein and OCA8-Related Mutant Variants.
Int J Mol Sci. 2022 Jan 24;23(3):1305. doi: 10.3390/ijms23031305.
4
Spectrophotometric Assays for Sensing Tyrosinase Activity and Their Applications.
Biosensors (Basel). 2021 Aug 23;11(8):290. doi: 10.3390/bios11080290.
5
Characterization of Temperature-Dependent Kinetics of Oculocutaneous Albinism-Causing Mutants of Tyrosinase.
Int J Mol Sci. 2021 Jul 21;22(15):7771. doi: 10.3390/ijms22157771.
6
Homology modeling and global computational mutagenesis of human myosin VIIa.
J Anal Pharm Res. 2021;10(1):41-48. doi: 10.15406/japlr.2021.10.00364. Epub 2021 Mar 4.
7
Histidine residues at the copper-binding site in human tyrosinase are essential for its catalytic activities.
J Enzyme Inhib Med Chem. 2020 Dec;35(1):726-732. doi: 10.1080/14756366.2020.1740691.
8
Human Tyrosinase: Temperature-Dependent Kinetics of Oxidase Activity.
Int J Mol Sci. 2020 Jan 30;21(3):895. doi: 10.3390/ijms21030895.
9
FlexiBAC: a versatile, open-source baculovirus vector system for protein expression, secretion, and proteolytic processing.
BMC Biotechnol. 2019 Mar 29;19(1):20. doi: 10.1186/s12896-019-0512-z.
10
Membrane-associated human tyrosinase is an enzymatically active monomeric glycoprotein.
PLoS One. 2018 Jun 5;13(6):e0198247. doi: 10.1371/journal.pone.0198247. eCollection 2018.
本文引用的文献
1
Oculocutaneous albinism type 1: link between mutations, tyrosinase conformational stability, and enzymatic activity.
Pigment Cell Melanoma Res. 2017 Jan;30(1):41-52. doi: 10.1111/pcmr.12546.
2
Albinism-causing mutations in recombinant human tyrosinase alter intrinsic enzymatic activity.
PLoS One. 2014 Jan 2;9(1):e84494. doi: 10.1371/journal.pone.0084494. eCollection 2014.
3
DNA variations in oculocutaneous albinism: an updated mutation list and current outstanding issues in molecular diagnostics.
Hum Mutat. 2013 Jun;34(6):827-35. doi: 10.1002/humu.22315. Epub 2013 Apr 30.
4
New insights into the active site structure and catalytic mechanism of tyrosinase and its related proteins.
Pigment Cell Melanoma Res. 2009 Dec;22(6):750-60. doi: 10.1111/j.1755-148X.2009.00636.x. Epub 2009 Sep 7.
5
Oculocutaneous albinism.
Orphanet J Rare Dis. 2007 Nov 2;2:43. doi: 10.1186/1750-1172-2-43.
6
Involvement of a novel copper chaperone in tyrosinase activity and melanin synthesis in Marinomonas mediterranea.
Microbiology (Reading). 2007 Jul;153(Pt 7):2241-2249. doi: 10.1099/mic.0.2007/006833-0.
7
Temperature sensitive oculocutaneous albinism associated with missense changes in the tyrosinase gene.
Br J Ophthalmol. 2005 Oct;89(10):1383-4. doi: 10.1136/bjo.2005.070243.
8
Tyrosinase inhibitor isolated from the leaves of Zanthoxylum piperitum.
Biosci Biotechnol Biochem. 2004 Sep;68(9):1984-7. doi: 10.1271/bbb.68.1984.
9
Molecular anatomy of tyrosinase and its related proteins: beyond the histidine-bound metal catalytic center.
Pigment Cell Res. 2002 Jun;15(3):162-73. doi: 10.1034/j.1600-0749.2002.02012.x.
10
Functional analysis of tyrosinase isozymes of cultured malignant melanoma cells during the recovery period following interrupted melanogenesis induced by glycosylation inhibitors.
J Invest Dermatol. 1984 Sep;83(3):196-201. doi: 10.1111/1523-1747.ep12263562.
Zotero 插件