Institute of Marine and Environmental Technology, University of Maryland Center for Environmental Science, 701 E. Pratt Street Columbus Center, Baltimore, MD 21202, USA.
Mar Drugs. 2017 Aug 1;15(8):241. doi: 10.3390/md15080241.
The insulin-like androgenic gland hormone (IAG) found in decapod crustaceans is known to regulate sexual development in males. IAG is produced in the male-specific endocrine tissue, the androgenic gland (AG); however, IAG expression has been also observed in other tissues of decapod crustacean species including and . This study aimed to isolate the full-length cDNA sequence of IAG from the AG of male red deep-sea crabs, (), and to examine its tissue distribution. To this end, we employed polymerase chain reaction cloning with degenerate primers and 5' and 3' rapid amplification of cDNA ends (RACE). The full-length cDNA sequence (1555 nt) includes a 366 nt 5' untranslated region a 453 nt open reading frame encoding 151 amino acids, and a relatively long 3' UTR of 733 nt. The ORF consists of a 19 aa signal peptide, 32 aa B chain, 56 aa C chain, and 44 aa A chain. The putative ChqIAG amino acid sequence is most similar to those found in other crab species, including and , which are clustered together phylogenetically.
在十足目甲壳动物中发现的胰岛素样雄激素腺激素 (IAG) 已知可调节雄性的性发育。IAG 是在雄性特有的内分泌组织——雄激素腺 (AG) 中产生的；然而，IAG 的表达也在其他十足目甲壳动物物种的组织中观察到，包括 和 。本研究旨在从雄性红深海蟹 ( ) 的 AG 中分离 IAG 的全长 cDNA 序列，并检查其组织分布。为此，我们使用了带有简并引物和 5'和 3'快速扩增 cDNA 末端 (RACE) 的聚合酶链反应克隆。全长 cDNA 序列 (1555nt) 包括一个 366nt 的 5'非翻译区、一个编码 151 个氨基酸的 453nt 开放阅读框和一个相对较长的 733nt 3'UTR。ORF 由 19 个氨基酸的信号肽、32 个氨基酸的 B 链、56 个氨基酸的 C 链和 44 个氨基酸的 A 链组成。推定的 ChqIAG 氨基酸序列与其他蟹种最相似，包括 和 ，它们在系统发育上聚类在一起。
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