College of Veterinary Medicine, Nanjing Agriculture University, Nanjing, 210095, China.
Ministry of Education Key Laboratory of Systems Biomedicine, Shanghai Center for Systems Biomedicine, and School of Pharmacy, Shanghai Jiao Tong University, Shanghai, 200240, China.
Sci Rep. 2017 Aug 1;7(1):7020. doi: 10.1038/s41598-017-06017-1.
The protective effects of Kisspeptin on heat-induced oxidative stress in rats were investigated by using a combination of biochemical parameters and metabonomics. Metabonomic analyses were performed using gas chromatography/mass spectrometry in conjunction with multivariate and univariate statistical analyses. At the end point of the heat stress experiment, histological observation, ultrastructural analysis and biochemical parameters were measured. Metabonomic analysis of liver tissue revealed that Kisspeptin mainly attenuated the alteration of purine metabolism and fatty acid metabolism pathways. Futhermore, Kisspeptin also increased the levels of GSH, T-AOC as well as SOD activities, and upregulated MDA levels. These results provide important mechanistic insights into the protective effects of Kisspeptin against heat-induced oxidative stress.
本研究采用生化参数与代谢组学相结合的方法，探讨 Kisspeptin 对热应激诱导的大鼠氧化应激的保护作用。代谢组学分析采用气相色谱/质谱联用技术，并结合多元和单变量统计分析。在热应激实验终点，进行组织学观察、超微结构分析和生化参数测量。肝组织代谢组学分析表明，Kisspeptin 主要减轻嘌呤代谢和脂肪酸代谢途径的改变。此外，Kisspeptin 还增加了 GSH、T-AOC 和 SOD 活性水平，并上调了 MDA 水平。这些结果为 Kisspeptin 对抗热应激诱导的氧化应激的保护作用提供了重要的机制见解。
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