剖析氯离子-硝酸根阴离子转运测定法。
Dissecting the chloride-nitrate anion transport assay.
作者信息
Yang Yufeng, Wu Xin, Busschaert Nathalie, Furuta Hiroyuki, Gale Philip A
机构信息
Chemistry, University of Southampton, Southampton, SO17 1BJ, UK and Department of Chemistry and Biochemistry, Kyushu University, Fukuoka, 819-0395, Japan.
School of Chemistry, The University of Sydney, NSW 2006, Australia.
出版信息
Chem Commun (Camb). 2017 Aug 25;53(66):9230-9233. doi: 10.1039/c7cc04912a. Epub 2017 Aug 2.
A systematic study of chloride vs. nitrate selectivity across six anion transporters has revealed a good correlation between the selectivities of their anion binding and membrane transport properties. This work reveals the limitations of the chloride-nitrate exchange assay and shows how new approaches can be used to measure anion uniport.
一项针对六种阴离子转运蛋白对氯离子与硝酸根离子选择性的系统研究表明，其阴离子结合选择性与膜转运特性之间存在良好的相关性。这项工作揭示了氯离子-硝酸根离子交换测定法的局限性，并展示了如何使用新方法来测量阴离子单向运输。
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