Department of Biochemical Engineering, Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10000, Zagreb, Croatia.
Bioprocess Biosyst Eng. 2017 Nov;40(11):1679-1688. doi: 10.1007/s00449-017-1823-x. Epub 2017 Aug 2.
Fossil fuels are still major energy sources, but the search for renewable energy sources has been encouraged. Bioethanol has been recognized as an alternative to fossil fuels and nowadays it represents more than 90% of the global biofuel production. Bioethanol production from raw sugar beet cossettes as a semi-solid substrate was studied. The study was carried out in the horizontal rotating tubular bioreactor (HRTB) with Saccharomyces cerevisiae as a microbial production strain. The impact of different combinations of HRTB operational parameters such as, rotation speed (5-15 min), rotation type [constant or interval (3-15 min h)] and working volume (ratio V /V = 0.2-0.7) on the bioethanol production was examined. In this study, the highest bioprocess efficiency parameters ([Formula: see text] = 0.47 g g, E = 87.36% and Pr = 0.618 g L h) were observed at 0.20 V /V , interval rotation of 12 min h and rotation speed of 15 min. It has to be pointed out that bioethanol production efficiency in the HRTB was on the similar level as observed by bioethanol production from the raw sugar beet juice. Naturally present microorganisms of sugar beet could have a significant impact on bioethanol production. Higher yeast inoculation rate could reduce contaminant activities and, consequently, the bioethanol production efficiency would be improved.
化石燃料仍是主要能源,但人们已经在鼓励寻找可再生能源。生物乙醇已被视为化石燃料的替代品,如今它占全球生物燃料产量的 90%以上。本文研究了以原糖甜菜碎屑为半固态基质生产生物乙醇。研究在水平旋转管式生物反应器(HRTB)中进行,使用酿酒酵母作为微生物生产菌株。考察了 HRTB 操作参数(转速 5-15 min、转速类型[恒速或间歇(3-15 min h)]和工作体积比(V/V = 0.2-0.7)的不同组合对生物乙醇生产的影响。在这项研究中,在 0.20 V/V、间歇旋转 12 min h 和转速 15 min 的条件下,观察到最高的生物过程效率参数([Formula: see text] = 0.47 g g、E = 87.36%和 Pr = 0.618 g L h)。需要指出的是,HRTB 中的生物乙醇生产效率与从原糖甜菜汁中生产生物乙醇时观察到的效率相当。甜菜中天然存在的微生物可能对生物乙醇生产产生重大影响。提高酵母接种率可以降低污染物的活性,从而提高生物乙醇的生产效率。