多面体低聚倍半硅氧烷（POSS）共递送时阿霉素活性的异常增强
Unusual Enhancement of Doxorubicin Activity on Co-Delivery with Polyhedral Oligomeric Silsesquioxane (POSS).
作者信息
Sobierajska Ewelina, Konopka Malgorzata, Janaszewska Anna, Piorecka Kinga, Blauz Andrzej, Klajnert-Maculewicz Barbara, Stanczyk Maciej, Stanczyk Wlodzimierz A
机构信息
Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, Pomorska 141/143, 90-236 Lodz, Poland.
Centre of Molecular and Macromolecular Studies, Polish Academy of Sciences, Sienkiewicza 112, 90-363 Lodz, Poland.
出版信息
Materials (Basel). 2017 May 20;10(5):559. doi: 10.3390/ma10050559.
Polyhedral oligomeric silsesquioxane (POSS), bearing eight 3-chloroammoniumpropyl substituents, was studied as a potential nanocarrier in co-delivery systems with doxorubicin (DOX). The toxicity of doxorubicin and POSS:DOX complexes at four different molar ratios (1:1; 1:2, 1:4, 1:8) towards microvascular endothelial cells (HMEC-1), breast cancer cells (MCF-7), and human cervical cancer endothelial cells (HeLa) was determined. The rate of penetration of the components into the cells, their cellular localization and the hydrodynamic diameter of the complexes was also determined. A cytotoxicity profile of POSS:DOX complexes indicated that the POSS:DOX system at the molar ratio of 1:8 was more effective than free DOX. Confocal images showed that DOX co-delivery with POSS allowed for more effective penetration of doxorubicin through the cell membrane. Taking all the results into account, it can be claimed that the polyhedral oligomeric silsesquioxane (T₈-POSS) is a promising, complex nanocarrier for doxorubicin delivery.
带有八个3-氯铵丙基取代基的多面体低聚倍半硅氧烷（POSS），作为与阿霉素（DOX）共递送系统中的潜在纳米载体进行了研究。测定了阿霉素和POSS:DOX复合物在四种不同摩尔比（1:1；1:2、1:4、1:8）下对微血管内皮细胞（HMEC-1）、乳腺癌细胞（MCF-7）和人宫颈癌内皮细胞（HeLa）的毒性。还测定了各组分进入细胞的速率、它们在细胞内的定位以及复合物的流体动力学直径。POSS:DOX复合物的细胞毒性谱表明，摩尔比为1:8的POSS:DOX系统比游离DOX更有效。共聚焦图像显示，DOX与POSS共递送能使阿霉素更有效地穿透细胞膜。综合所有结果，可以认为多面体低聚倍半硅氧烷（T₈-POSS）是一种用于阿霉素递送的有前景的复合纳米载体。
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