O'Daly J A, Cabrera Z
Acta Trop. 1986 Sep;43(3):225-36.
Hamsters immunized with N-p-tosyl-L-lysine-chloromethyl ketone TLCK-treated L. brasiliensis brasiliensis (LB) from culture, infected with LB amastigotes presented: a gradual increase in T and B cell responsiveness to mitogens by lymph node lymphocytes, and an increased response to concanavalin A with no changes for dextran sulphate and pokeweed mitogen in splenocytes. Absence of parasites in lymph nodes after 6 weeks post-infection and a nodule 4 times smaller than that of infected control animals. The nodule was undetectable after 70 days of infection. Hamsters preimmunized with TLCK-treated L. donovani (LD) from culture did not show suppression of the blastogenic response to mitogens of spleen and lymph node cells after infection with LD amastigotes and survived for more than one year, whereas infected, unimmunized animals died five months after infection. Animals preimmunized with culture parasites (LB or LD) treated with phenyl-methyl-sulphonyl-fluoride (PMSF) and infected with LB or LD amastigotes did not show any protective effect.
用对甲苯磺酰-L-赖氨酸氯甲基酮（TLCK）处理过的来自培养物的巴西利什曼原虫巴西亚种（LB）免疫的仓鼠，感染巴西利什曼原虫无鞭毛体后表现出：淋巴结淋巴细胞对丝裂原的T细胞和B细胞反应性逐渐增加，脾细胞对刀豆球蛋白A的反应增加，而对硫酸葡聚糖和商陆有丝分裂原的反应无变化。感染后6周淋巴结中无寄生虫，且结节比感染对照动物的结节小4倍。感染70天后结节无法检测到。用TLCK处理过的来自培养物的杜氏利什曼原虫（LD）预免疫的仓鼠，在感染杜氏利什曼原虫无鞭毛体后，脾细胞和淋巴结细胞对丝裂原的增殖反应未受到抑制，存活超过一年，而未免疫的感染动物在感染后五个月死亡。用苯甲基磺酰氟（PMSF）处理过的培养寄生虫（LB或LD）预免疫并感染LB或LD无鞭毛体的动物未表现出任何保护作用。
