Chiu Chih-Hao, Lei Kin Fong, Yeh Wen-Ling
Bone and Joint Research Center, Department of Orthopedic Surgery, Linkou Chang Gung Memorial Hospital, Taoyuan, Taiwan.
Department of Orthopedic Surgery, Taoyuan Chang Gung Memorial Hospital, Taoyuan, Taiwan.
Biomed Microdevices. 2017 Sep;19(3):69. doi: 10.1007/s10544-017-0214-z.
One of the objectives of rotator cuff repairs is to achieve biological healing and recovery in the tendon-bone zone. Some clinical evaluations reported the feasibility of tendon healing based on the stimulations of electric field and platelet-rich plasma (PRP). However, because of lack of appropriate tool for in vitro primary culture under complicated conditions, the efficacy and standard protocol of these healing approaches are still controversial among clinical experts. In this study, a novel co-culture device was developed for the study of tenocytes proliferation under single and combined stimulations of electric field and PRP. The device was a culture well divided into three sub-chambers separated by a barrier and embedded with a pair of parallel plate electrodes. Tenocytes and PRP gel could be respectively loaded into the sub-chambers and cultured with interlinked medium. Hence, tenocytes could concurrently receive a uniform electric field and platelet-derived growth factors by diffusion. Results revealed that the proliferation of tenocytes could be significantly enhanced by these stimulations. The device provides a precise and practical approach for the in vitro study of tendon healing, especially for PRP study. Moreover, optimization of the conditions of electric field and PRP could be determined by in vitro screening procedure before surgery to provide a personalized therapy.
肩袖修复的目标之一是在肌腱-骨区域实现生物学愈合和恢复。一些临床评估报告了基于电场和富血小板血浆（PRP）刺激实现肌腱愈合的可行性。然而，由于缺乏在复杂条件下进行体外原代培养的合适工具，这些愈合方法的疗效和标准方案在临床专家中仍存在争议。在本研究中，开发了一种新型共培养装置，用于研究电场和PRP单一及联合刺激下的肌腱细胞增殖。该装置是一个培养孔，分为三个子腔室，由一个屏障隔开，并嵌入一对平行板电极。肌腱细胞和PRP凝胶可分别加载到子腔室中，并通过相互连接的培养基进行培养。因此，肌腱细胞可以通过扩散同时接受均匀的电场和血小板衍生生长因子。结果显示，这些刺激可显著增强肌腱细胞的增殖。该装置为肌腱愈合的体外研究，特别是PRP研究，提供了一种精确而实用的方法。此外，电场和PRP条件的优化可以在手术前通过体外筛选程序来确定，以提供个性化治疗。
