谷氨酰胺对多变鱼腥藻生长和异形胞分化的影响。
Effect of glutamine on growth and heterocyst differentiation in the cyanobacterium Anabaena variabilis.
作者信息
Thiel T, Leone M
出版信息
J Bacteriol. 1986 Nov;168(2):769-74. doi: 10.1128/jb.168.2.769-774.1986.
Abstract
Mutants of the cyanobacterium Anabaena variabilis that were capable of increased uptake of glutamine, as compared with that in the parental strains, were isolated. Growth of these mutants and their parental strains was measured in media containing N2, ammonia, or glutamine as a source of nitrogen. All strains grew well with any one of these sources of fixed nitrogen. Much of the glutamine taken up by the cells was converted to glutamate. The concentrations of glutamine, glutamate, arginine, ornithine, and citrulline in free amino acid pools in glutamine-grown cells were high compared with the concentrations of these amino acids in ammonia-grown or N2-grown cells. All strains capable of heterocyst differentiation, including a strain which produced nonfunctional heterocysts, grew and formed heterocysts in the presence of glutamine. However, nitrogenase activity was repressed in glutamine-grown cells. Glutamine may not be the molecule directly responsible for repression of the differentiation of heterocysts.
摘要
分离出了与亲本菌株相比能够增加谷氨酰胺摄取量的多变鱼腥蓝细菌突变体。在含有N₂、氨或谷氨酰胺作为氮源的培养基中测定了这些突变体及其亲本菌株的生长情况。所有菌株利用这些固定氮源中的任何一种都生长良好。细胞摄取的大部分谷氨酰胺都转化为了谷氨酸。与在以氨或N₂为氮源生长的细胞中这些氨基酸的浓度相比，在以谷氨酰胺为氮源生长的细胞中，游离氨基酸池中谷氨酰胺、谷氨酸、精氨酸、鸟氨酸和瓜氨酸的浓度较高。所有能够进行异形胞分化的菌株，包括产生无功能异形胞的菌株，在谷氨酰胺存在的情况下都能生长并形成异形胞。然而，在以谷氨酰胺为氮源生长的细胞中，固氮酶活性受到抑制。谷氨酰胺可能不是直接负责抑制异形胞分化的分子。
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