Andrews Sarah F, Joyce M Gordon, Chambers Michael J, Gillespie Rebecca A, Kanekiyo Masaru, Leung Kwanyee, Yang Eun Sung, Tsybovsky Yaroslav, Wheatley Adam K, Crank Michelle C, Boyington Jeffrey C, Prabhakaran Madhu S, Narpala Sandeep R, Chen Xuejun, Bailer Robert T, Chen Grace, Coates Emily, Kwong Peter D, Koup Richard A, Mascola John R, Graham Barney S, Ledgerwood Julie E, McDermott Adrian B
Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
Electron Microscopy Laboratory, Cancer Research Technology Program, Leidos Biomedical Research Inc., Frederick National Laboratory for Cancer Research, Frederick, MD 21701, USA.
Sci Immunol. 2017 Jul 14;2(13). doi: 10.1126/sciimmunol.aan2676.
Antigenic drift and shift of influenza strains underscore the need for broadly protective influenza vaccines. One strategy is to design immunogens that elicit B cell responses against conserved epitopes on the hemagglutinin (HA) stem. To better understand the elicitation of HA stem-targeted B cells to group 1 and group 2 influenza subtypes, we compared the memory B cell response to group 2 H7N9 and group 1 H5N1 vaccines in humans. Upon H7N9 vaccination, almost half of the HA stem-specific response recognized the group 1 and group 2 subtypes, whereas the response to H5N1 was largely group 1-specific. Immunoglobulin repertoire analysis of HA-specific B cells indicated that the H7N9 and H5N1 vaccines induced genetically similar cross-group HA stem-binding B cells, albeit at a much higher frequency upon H7N9 vaccination. These data suggest that a group 2-based stem immunogen could prove more effective than a group 1 immunogen at eliciting broad cross-group protection in humans.
流感毒株的抗原漂移和转换凸显了研发具有广泛保护作用的流感疫苗的必要性。一种策略是设计能引发针对血凝素（HA）茎部保守表位的B细胞应答的免疫原。为了更好地理解针对1组和2组流感亚型的HA茎部靶向B细胞的激发情况，我们比较了人类对2组H7N9和1组H5N1疫苗的记忆B细胞应答。接种H7N9疫苗后，几乎一半的HA茎部特异性应答可识别1组和2组亚型，而对H5N1的应答主要是1组特异性的。对HA特异性B细胞的免疫球蛋白库分析表明，H7N9和H5N1疫苗诱导出基因上相似的跨组HA茎部结合B细胞，尽管接种H7N9疫苗后其频率要高得多。这些数据表明，基于2组的茎部免疫原在引发人类广泛的跨组保护方面可能比基于1组的免疫原更有效。
Proc Natl Acad Sci U S A. 2025-2-18
Sci Immunol. 2025-1-24
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